Application | Comment | Organism |
---|---|---|
synthesis | protocol for overproduction of enzyme | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
E97D | reduction of both Km and kcat-value | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | assay methods for both directions of reaction | Methanosarcina thermophila | |
2.8 | - |
acetate | mutant E97D, pH 7.5, 37°C | Methanosarcina thermophila | |
22 | - |
acetate | wild-type, pH 7.5, 37°C | Methanosarcina thermophila | |
24 | - |
propionate | wild-type, pH 7.5, 37°C | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + hydroxylamine | - |
Methanosarcina thermophila | acetyl hydroxamate + phosphate | - |
r | |
ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
ATP + butyrate | 2% of reactivity with acetate | Methanosarcina thermophila | ADP + butyryl phosphate | - |
r | |
ATP + ethanol | 1% of reactivity with acetate | Methanosarcina thermophila | ADP + ethyl phosphate | - |
r | |
ATP + formate | 2% of reactivity with acetate | Methanosarcina thermophila | ADP + formyl phosphate | - |
r | |
ATP + glycerol | 1% of reactivity with acetate | Methanosarcina thermophila | ADP + glycerol phosphate | - |
r | |
ATP + glycine | 1% of reactivity with acetate | Methanosarcina thermophila | ADP + glycyl phosphate | - |
r | |
ATP + glycolic acid | 4% of reactivity with acetate | Methanosarcina thermophila | ADP + ? | - |
r | |
ATP + propionate | - |
Methanosarcina thermophila | ADP + propionyl phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
acetate | mutant E97D, pH 7.5, 37°C | Methanosarcina thermophila | |
1042 | - |
acetate | wild-type, pH 7.5, 37°C | Methanosarcina thermophila |