General Stability | Organism |
---|---|
destroyed by repeated freezing and thawing | Salmonella enterica subsp. enterica serovar Typhimurium |
destroyed by repeated freezing and thawing | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | - |
Escherichia coli | |
Li+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Na+ | - |
Escherichia coli | |
Na+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
propionic acid | competitive inhibitor with respect to acetate | Escherichia coli | |
propionic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.07 | - |
ATP | pH 7.9, 37°C | Escherichia coli | |
0.16 | - |
acetyl phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.16 | - |
acetyl phosphate | pH 7.9, 37°C | Escherichia coli | |
0.5 | - |
ADP | pH 7.9, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.5 | - |
ADP | pH 7.9, 37°C | Escherichia coli | |
0.78 | - |
ITP | pH 7.9, 37°C | Escherichia coli | |
0.94 | - |
ATP | pH 7.9, 37°C | Escherichia coli | |
1.1 | - |
GTP | pH 7.9, 37°C | Escherichia coli | |
7 | - |
acetate | pH 7.9, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
7 | - |
acetate | pH 7.9, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Escherichia coli |
41200 | - |
2 * 41200, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
68000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
70000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
ADP + acetyl phosphate | - |
r | |
ATP + acetate | Escherichia coli | - |
ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K235, 1100 thi- | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT2, wild-type | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
partial | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, relatively stable to storage when frozen, remains active for at least 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + acetyl phosphate | - |
r | |
ATP + acetate | - |
Escherichia coli | ADP + acetyl phosphate | - |
r | |
GTP + acetate | - |
Escherichia coli | GDP + acetyl phosphate | - |
r | |
ITP + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | IDP + acetyl phosphate | - |
r | |
ITP + acetate | - |
Escherichia coli | IDP + acetyl phosphate | - |
r | |
additional information | neither propionic acid nor butyric acid are active as substrates | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | neither propionic acid nor butyric acid are active as substrates | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Escherichia coli |
dimer | 2 * 41200, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
7.5 | - |
- |
Escherichia coli |