BRENDA - Enzyme Database show
show all sequences of 2.7.2.1

Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima

Bock, A.K.; Glasemacher, J.; Schmidt, R.; Schonheit, P.; J. Bacteriol. 181, 1861-1867 (1999)

Data extracted from this reference:

General Stability
General Stability
Organism
stabilized against heat inactivation by salts, most effective (NH4)2SO4
Thermotoga maritima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.44
-
acetyl phosphate
pH 7.0, 50°C
Thermotoga maritima
0.7
-
ATP
pH 7.0, 50°C
Thermotoga maritima
3
-
ADP
pH 7.0, 50°C
Thermotoga maritima
40
-
acetate
pH 7.0, 50°C
Thermotoga maritima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Thermotoga maritima
Co2+
-
Thermotoga maritima
Mg2+
-
Thermotoga maritima
Mn2+
-
Thermotoga maritima
Ni2+
-
Thermotoga maritima
Zn2+
-
Thermotoga maritima
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
2 * 44000, alpha2 homodimer, SDS-PAGE
Thermotoga maritima
90000
-
gel filtration
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + acetate
Thermotoga maritima
-
ADP + acetyl phosphate
-
Thermotoga maritima
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
-
-
-
Oxidation Stability
Oxidation Stability
Organism
not sensitive to oxygen
Thermotoga maritima
Purification (Commentary)
Commentary
Organism
-
Thermotoga maritima
Storage Stability
Storage Stability
Organism
-20°C, can be stored in 20 mM Tris-HCl, pH 8.0, 2.0 mM MgCl2, 0.32 M NaCl, supplemented with 10% glycerol, without significant loss of activity for several weeks
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + acetate
-
487537
Thermotoga maritima
ADP + acetyl phosphate
-
487537
Thermotoga maritima
r
ATP + propionate
-
487537
Thermotoga maritima
ADP + propionyl phosphate
-
487537
Thermotoga maritima
r
CTP + acetate
-
487537
Thermotoga maritima
CDP + acetyl phosphate
-
487537
Thermotoga maritima
r
GTP + acetate
-
487537
Thermotoga maritima
GDP + acetyl phosphate
-
487537
Thermotoga maritima
r
ITP + acetate
-
487537
Thermotoga maritima
IDP + acetyl phosphate
-
487537
Thermotoga maritima
r
additional information
butyrate, isobutyrate, valerate and isovalerate are no substrates
487537
Thermotoga maritima
?
-
-
-
-
UTP + acetate
-
487537
Thermotoga maritima
UDP + acetyl phosphate
-
487537
Thermotoga maritima
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 44000, alpha2 homodimer, SDS-PAGE
Thermotoga maritima
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Thermotoga maritima
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
100
activity increases rapidly above 55°C, enzyme does not lose activity at 80°C upon incubation for 180 min
Thermotoga maritima
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
100
almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4
Thermotoga maritima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Thermotoga maritima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
about 50% activity in this range
Thermotoga maritima
General Stability (protein specific)
General Stability
Organism
stabilized against heat inactivation by salts, most effective (NH4)2SO4
Thermotoga maritima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.44
-
acetyl phosphate
pH 7.0, 50°C
Thermotoga maritima
0.7
-
ATP
pH 7.0, 50°C
Thermotoga maritima
3
-
ADP
pH 7.0, 50°C
Thermotoga maritima
40
-
acetate
pH 7.0, 50°C
Thermotoga maritima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Thermotoga maritima
Co2+
-
Thermotoga maritima
Mg2+
-
Thermotoga maritima
Mn2+
-
Thermotoga maritima
Ni2+
-
Thermotoga maritima
Zn2+
-
Thermotoga maritima
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
2 * 44000, alpha2 homodimer, SDS-PAGE
Thermotoga maritima
90000
-
gel filtration
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + acetate
Thermotoga maritima
-
ADP + acetyl phosphate
-
Thermotoga maritima
r
Oxidation Stability (protein specific)
Oxidation Stability
Organism
not sensitive to oxygen
Thermotoga maritima
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermotoga maritima
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, can be stored in 20 mM Tris-HCl, pH 8.0, 2.0 mM MgCl2, 0.32 M NaCl, supplemented with 10% glycerol, without significant loss of activity for several weeks
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + acetate
-
487537
Thermotoga maritima
ADP + acetyl phosphate
-
487537
Thermotoga maritima
r
ATP + propionate
-
487537
Thermotoga maritima
ADP + propionyl phosphate
-
487537
Thermotoga maritima
r
CTP + acetate
-
487537
Thermotoga maritima
CDP + acetyl phosphate
-
487537
Thermotoga maritima
r
GTP + acetate
-
487537
Thermotoga maritima
GDP + acetyl phosphate
-
487537
Thermotoga maritima
r
ITP + acetate
-
487537
Thermotoga maritima
IDP + acetyl phosphate
-
487537
Thermotoga maritima
r
additional information
butyrate, isobutyrate, valerate and isovalerate are no substrates
487537
Thermotoga maritima
?
-
-
-
-
UTP + acetate
-
487537
Thermotoga maritima
UDP + acetyl phosphate
-
487537
Thermotoga maritima
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 44000, alpha2 homodimer, SDS-PAGE
Thermotoga maritima
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Thermotoga maritima
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
100
activity increases rapidly above 55°C, enzyme does not lose activity at 80°C upon incubation for 180 min
Thermotoga maritima
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
100
almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4
Thermotoga maritima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Thermotoga maritima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8.5
about 50% activity in this range
Thermotoga maritima
Other publictions for EC 2.7.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730304
Ingram-Smith
The role of active site residu ...
Methanosarcina thermophila
Life
5
861-871
2015
-
-
1
-
7
-
-
29
-
-
-
-
-
2
-
-
1
-
-
-
-
-
6
-
2
-
-
23
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
29
-
-
-
-
-
-
-
1
-
-
-
-
6
-
2
-
-
23
-
-
-
-
-
-
-
-
22
22
737465
Rozova
Acetate kinase-an enzyme of th ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Antonie van Leeuwenhoek
108
965-974
2015
-
-
1
-
-
-
-
5
-
4
2
2
-
8
-
-
1
-
-
-
-
-
4
1
1
1
1
4
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
5
-
4
2
2
-
-
-
1
-
-
-
-
4
1
1
1
1
4
1
1
-
-
-
1
1
-
-
-
738863
Zhang
Enzymatic manufacture of deoxy ...
Escherichia coli
J. Microbiol. Biotechnol.
25
2034-2042
2015
-
-
1
-
1
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
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1
2
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1
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-
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-
1
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1
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-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
746769
Rozova
Acetate kinase-an enzyme of t ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Antonie van Leeuwenhoek
108
965-974
2015
-
-
1
-
-
-
-
2
-
3
3
-
-
8
-
-
1
-
-
-
-
-
4
1
1
2
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
3
3
-
-
-
-
1
-
-
-
-
4
1
1
2
1
2
1
-
-
-
-
-
-
-
2
2
727558
Welte
Experimental evidence of an ac ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEMS Microbiol. Lett.
359
147-153
2014
-
-
1
-
-
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
738528
Puri
Regulation of acetate kinase i ...
Lactococcus lactis, Lactococcus lactis MG1363
J. Bacteriol.
196
1386-1393
2014
-
-
1
-
-
-
4
8
-
3
-
4
-
5
-
-
1
-
-
-
-
-
4
-
1
-
-
4
1
-
-
2
7
-
-
-
-
2
4
-
-
-
-
8
11
10
-
6
-
4
-
-
-
2
-
-
-
-
4
-
2
-
-
4
2
-
-
-
-
1
2
-
-
-
738717
Yoshioka
Structural and mutational anal ...
Escherichia coli
J. Biosci. Bioeng.
118
502-507
2014
-
-
1
-
7
-
-
8
-
1
-
1
-
3
-
-
1
-
-
-
-
-
3
-
1
-
-
7
1
-
-
3
-
-
-
-
-
1
3
-
7
-
-
-
-
8
-
1
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
7
1
-
-
-
-
2
2
-
-
-
739218
Kushkevych
Acetate kinase activity and ki ...
Desulfomicrobium sp., Desulfomicrobium sp. Rod-9, Desulfovibrio piger, Desulfovibrio piger Vib-7
Open Microbiol. J.
8
138-143
2014
-
-
-
-
-
-
-
2
2
2
-
4
-
16
-
-
-
-
-
2
2
-
4
-
2
2
-
-
2
2
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
2
2
2
-
4
-
-
-
-
-
2
2
-
4
-
2
2
-
-
2
2
-
-
-
2
2
-
-
-
739591
Tang
Cold adaptation: structural an ...
Escherichia coli, Shewanella sp., Shewanella sp. AS-11
Protein J.
33
313-322
2014
-
-
2
-
-
-
-
30
-
3
6
3
-
6
-
-
2
-
-
-
-
-
7
2
2
-
2
32
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
30
-
3
6
3
-
-
-
2
-
-
-
-
7
2
2
-
2
32
2
-
-
-
-
5
5
-
32
32
723068
Thaker
Crystal structures of acetate ...
Cryptococcus neoformans, Entamoeba histolytica
J. Struct. Biol.
181
185-189
2013
-
-
2
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
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2
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2
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-
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-
-
-
-
738854
Asgari
Targeting acetate kinase: inhi ...
Escherichia coli
J. Microbiol. Biotechnol.
23
1544-1553
2013
-
-
-
-
-
-
3
-
-
1
-
1
-
3
-
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-
-
-
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1
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-
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2
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2
-
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3
-
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-
1
-
1
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721949
Chittori
Structural and mechanistic inv ...
Salmonella enterica subsp. enterica serovar Typhimurium
BMC Struct. Biol.
12
24
2012
-
-
1
1
-
-
5
11
-
4
1
-
-
4
-
-
1
-
-
-
-
1
6
-
1
1
-
8
1
1
-
-
-
-
5
-
-
1
-
1
-
-
5
5
-
11
-
4
1
-
-
-
-
1
-
-
-
1
6
-
1
1
-
8
1
1
-
-
-
-
-
-
8
8
721205
Chittori
Preliminary X-ray crystallogra ...
Salmonella enterica subsp. enterica serovar Typhimurium
Acta Crystallogr. Sect. F
67
1658-1661
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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-
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-
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1
-
1
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-
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-
722528
Ramos-Montanez
Instability of ackA (acetate k ...
Streptococcus pneumoniae
J. Bacteriol.
192
6390-6400
2010
-
-
-
-
-
-
-
-
-
-
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-
-
4
-
-
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-
-
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-
-
-
-
-
-
1
1
-
-
-
691204
Mukhopadhyay
A continuous assay of acetate ...
Thermotoga maritima
Bioorg. Chem.
36
65-69
2008
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
673209
Ren
Cloning, expression, and chara ...
Ethanoligenens harbinense
Curr. Microbiol.
55
167-172
2007
-
-
1
-
-
-
-
-
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1
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4
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1
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1
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1
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1
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1
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1
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1
1
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1
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1
1
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1
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1
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1
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-
673750
Baldock
Roseovarius sp. strain 217: ae ...
Roseovarius sp., Roseovarius sp. 217
FEMS Microbiol. Lett.
271
202-206
2007
-
-
-
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690888
Gorrell
Investigation of the Methanosa ...
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22
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22
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2
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12
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Transcription in Mycoplasma pn ...
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2
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Construction and characterizat ...
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1
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677237
Ingram-Smith
Acetate kinase: not just a bac ...
Aspergillus fumigatus, Aspergillus nidulans, Bacillus subtilis, Borreliella burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Entamoeba histolytica, Enterococcus faecalis, Fusarium graminearum, Helicobacter pylori, Histoplasma capsulatum, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Methanosarcina thermophila, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Parastagonospora nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Trichoderma reesei, Uncinocarpus reesii, Ustilago maydis
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37
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37
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Characterization of the acetat ...
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5
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4
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21
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5
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27
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4
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21
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659446
Gorrell
Structural and kinetic analyse ...
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13
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1
32
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2
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13
-
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659856
Iyer
-
Acetate kinase from Methanosar ...
Methanosarcina thermophila
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2005
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1
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1
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4
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1
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9
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2
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1
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1
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4
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1
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9
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2
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Hasona
Pyruvate formate lyase and ace ...
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642198
Ito
Highly sensitive simultaneous ...
Geobacillus stearothermophilus
Anal. Sci.
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2003
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727342
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Cloning of a gene encoding ace ...
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1
1
1
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1
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2
2
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4
1
1
1
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1
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727839
Miles
Evidence for a transition stat ...
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2002
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11
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1
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8
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1
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11
8
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1
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1
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1
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Cloning, expression, and chara ...
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2001
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1
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1
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1
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1
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4
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2
1
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1
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1
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2
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1
1
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642196
Miles
Site-directed mutational analy ...
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11
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28
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9
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1
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1
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11
-
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28
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3
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1
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9
-
1
-
-
-
-
-
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642197
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Purification and characterizat ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F.
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411-421
2001
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1
1
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1
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2
1
2
1
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1
1
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1
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1
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1
3
2
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1
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2
1
2
1
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1
1
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727737
Buss
Urkinase: structure of acetate ...
Methanosarcina thermophila
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680-686
2001
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1
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1
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1
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642193
Ingram-Smith
The role of histidines in the ...
Methanosarcina thermophila
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275
33765-33770
2000
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9
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1
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9
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1
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29
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1
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-
-
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642194
Singh-Wissmann
Identification of essential ar ...
Methanosarcina thermophila
Biochemistry
39
3671-3677
2000
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1
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7
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1
19
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1
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3
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1
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1
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1
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7
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1
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19
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1
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1
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1
-
-
-
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487537
Bock
Purification and characterizat ...
Thermotoga maritima
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1
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1
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1
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1
7
1
1
1
1
-
1
1
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-
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1
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4
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6
2
1
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1
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1
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1
7
1
1
1
1
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1
1
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-
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642190
Lin
-
Purification and characterizat ...
Hungateiclostridium thermocellum
Tetrahedron
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15915-15925
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1
1
1
1
1
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1
1
1
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1
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1
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2
2
1
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1
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1
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1
1
1
1
1
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1
1
1
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642191
Singh-Wissmann
Identification of essential gl ...
Methanosarcina thermophila
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1998
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1
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1
1
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1
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3
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2
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21
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1
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-
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1
-
1
1
-
-
-
-
-
-
-
-
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-
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642188
Buss
Crystallization of acetate kin ...
Methanosarcina thermophila
Protein Sci.
6
2659-2662
1997
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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642189
Winzer
-
Acetate kinase from Clostridiu ...
Clostridium acetobutylicum
Microbiology
143
3279-3286
1997
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1
-
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2
4
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4
3
1
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1
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1
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7
1
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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2
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4
-
4
3
1
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1
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1
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7
1
-
-
-
-
-
-
-
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642187
Diez-Gonzalez
The acetate kinase of Clostrid ...
Clostridium acetobutylicum, Clostridium acetobutylicum P262
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166
418-420
1996
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1
3
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1
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4
1
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4
1
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1
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3
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2
2
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1
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1
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4
1
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4
1
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642184
Aceti
-
Purification and characterizat ...
Methanosarcina thermophila
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15444-15448
1988
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2
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2
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2
1
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1
1
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1
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1
1
8
1
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1
1
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1
1
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-
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1
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-
-
-
-
-
-
2
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-
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2
1
4
2
1
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1
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1
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1
1
8
1
-
1
1
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1
1
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1
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642185
Kim
Inactivation of Acinetobacter ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
956
103-109
1988
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-
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1
2
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1
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2
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1
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1
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-
-
-
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-
-
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-
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-
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1
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2
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
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642186
Mannens
-
Purification and immobilizatio ...
Desulfovibrio vulgaris
Biotechnol. Lett.
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563-568
1988
-
-
-
-
-
-
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1
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1
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1
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1
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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1
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1
-
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1
-
1
-
-
-
-
-
-
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727804
Aceti
Purification and characterizat ...
Methanosarcina thermophila, Methanosarcina thermophila TM-1
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263
15444-15448
1988
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1
1
2
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3
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1
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1
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1
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1
14
1
1
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1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
4
3
2
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1
-
1
-
1
-
1
14
1
1
-
1
-
1
1
-
1
-
1
1
-
-
-
642182
Fox
Isolation and characterization ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
261
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1986
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2
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1
10
2
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-
-
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2
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-
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-
-
-
-
-
-
2
-
6
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11
-
-
4
3
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-
-
2
-
-
-
1
10
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
642183
Vigenschow
Purification and properties of ...
Rhodopseudomonas palustris
Biol. Chem. Hoppe-Seyler
367
951-956
1986
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11
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2
4
1
-
2
-
-
1
-
-
-
1
2
8
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
11
-
2
4
1
-
-
-
1
-
-
1
2
8
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
642181
Ichikawa
-
Purification and properties of ...
Propionibacterium freudenreichii
J. Ferment. Technol.
63
143-149
1985
-
-
-
-
-
-
-
6
-
6
2
1
-
1
-
-
1
-
-
-
1
-
5
1
1
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
6
2
1
-
-
-
1
-
-
1
-
5
1
1
-
1
-
2
1
1
-
-
-
-
-
-
-
642179
Harwood
Properties of acetate kinase i ...
Spirochaeta sp., Spirochaeta sp. MA-2
J. Bacteriol.
152
246-254
1982
-
-
-
-
-
-
-
6
-
6
2
2
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
6
2
2
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642180
Suzuki
-
Acetate kinase from Bacillus s ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503
Methods Enzymol.
90
179-185
1982
3
-
-
1
-
-
-
-
-
3
2
2
-
13
-
-
1
-
-
-
1
-
10
1
-
-
1
-
1
-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
3
2
2
-
-
-
1
-
-
1
-
10
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
642178
Nishimura
-
Acetate kinase from Veilonella ...
Veillonella parvula, Veillonella parvula 221 / ATCC 17745
Methods Enzymol.
71
311-316
1981
1
-
-
-
-
-
-
-
-
3
2
2
-
2
-
-
1
-
-
-
1
1
10
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
2
2
-
-
-
1
-
-
1
1
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642177
Wong
Inactivation of Escherichia co ...
Escherichia coli
Biochim. Biophys. Acta
615
121-131
1980
-
-
-
-
-
1
3
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642174
Griffith
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
254
442-446
1979
1
-
-
-
-
-
5
-
-
-
2
1
-
2
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
642175
Griffith
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
254
6698-6702
1979
1
-
-
-
-
-
2
4
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
642176
Kahane
Purification and properties of ...
Acholeplasma laidlawii, Acholeplasma laidlawii oral
J. Bacteriol.
137
764-772
1979
-
-
-
-
-
1
6
6
-
1
5
2
-
3
-
-
1
-
-
-
1
1
11
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
6
-
1
5
2
-
-
-
1
-
-
1
1
11
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
642172
Nakajima
Purification and properties of ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503
J. Biochem.
84
193-203
1978
-
-
-
1
-
1
2
-
-
6
3
2
-
14
1
-
1
-
-
-
1
-
11
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
6
3
2
-
1
-
1
-
-
1
-
11
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
642173
Yoshimura
Purification and characterizat ...
Veillonella parvula
Arch. Biochem. Biophys.
189
424-432
1978
1
-
-
-
-
-
9
4
-
5
2
1
-
3
-
-
1
-
-
-
1
2
5
1
-
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
9
-
4
-
5
2
1
-
-
-
1
-
-
1
2
5
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
642171
Bowman
Acetate kinase from Veillonell ...
Veillonella parvula
J. Biol. Chem.
251
3117-3121
1976
1
-
-
-
-
-
-
10
-
-
2
1
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
10
-
-
2
1
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642168
Janson
The inhibition of acetate, pyr ...
Escherichia coli
J. Biol. Chem.
249
2567-2571
1974
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642169
Janson
The specificity of chromium nu ...
Escherichia coli
J. Biol. Chem.
249
2572-2574
1974
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
642170
Schaupp
Purification and properties of ...
Moorella thermoacetica
Arch. Microbiol.
100
121-129
1974
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
642167
Anthony
Exchange reactions catalyzed b ...
Escherichia coli
J. Biol. Chem.
246
6129-6135
1971
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
642166
Rose
-
Acetate kinase ...
Escherichia coli, Lactobacillus delbrueckii, Streptococcus pyogenes
The Enzymes, 2nd. Ed. (Boyer, P. D. , Lardy, H. , Myrbaeck, K. , eds. )
6
115-118
1962
-
-
-
-
-
1
3
4
-
1
-
3
-
3
-
-
2
-
-
-
1
-
7
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
3
-
4
-
1
-
3
-
-
-
2
-
-
1
-
7
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-