Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.13.3 extracted from

  • Wang, D.; Chen, W.; Huang, S.; He, Y.; Liu, X.; Hu, Q.; Wei, T.; Sang, H.; Gan, J.; Chen, H.
    Structural basis of Zn(II) induced metal detoxification and antibiotic resistance by histidine kinase CzcS in Pseudomonas aeruginosa (2017), PLoS Pathog., 13, e1006533 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the CzcS sensor domain in complex with Zn(II) at 1.7 A resolution. The sensor domain is an alpha/beta-fold and senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues His55 and Asp60 on the dimer interface. The sensor domain interacts with the effector with the N-terminal alpha-helices rather than the conserved beta-sheets pocket. The dimerization of the N-terminal H1 and H1' alpha-helices is of primary importance for the activity of histidine kinase activity Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D60C contrary to wild-type, mutant strain displays Co(II)-inducible resistance to antibiotic meropenem Pseudomonas aeruginosa
H55A mutant loses its intrinsic resistance to Zn(II) and meropenem due to the destruction of Zn(II) binding site Pseudomonas aeruginosa
L38C/H55A mutation L38C restores the lost responsiveness of mutation H55A to Zn(II) stimulus Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information wild-type is not inducible by Co2+ Pseudomonas aeruginosa
Zn2+ the sensor domain of CzcS senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues His55 and Asp60 on the dimer interface Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I0V9
-
-
Pseudomonas aeruginosa DSM 22644 Q9I0V9
-
-

Synonyms

Synonyms Comment Organism
CzcS
-
Pseudomonas aeruginosa
PA2524
-
Pseudomonas aeruginosa
sensor protein czcS
-
Pseudomonas aeruginosa