Crystallization (Comment) | Organism |
---|---|
crystal structure of the CzcS sensor domain in complex with Zn(II) at 1.7 A resolution. The sensor domain is an alpha/beta-fold and senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues His55 and Asp60 on the dimer interface. The sensor domain interacts with the effector with the N-terminal alpha-helices rather than the conserved beta-sheets pocket. The dimerization of the N-terminal H1 and H1' alpha-helices is of primary importance for the activity of histidine kinase activity | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
D60C | contrary to wild-type, mutant strain displays Co(II)-inducible resistance to antibiotic meropenem | Pseudomonas aeruginosa |
H55A | mutant loses its intrinsic resistance to Zn(II) and meropenem due to the destruction of Zn(II) binding site | Pseudomonas aeruginosa |
L38C/H55A | mutation L38C restores the lost responsiveness of mutation H55A to Zn(II) stimulus | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | wild-type is not inducible by Co2+ | Pseudomonas aeruginosa | |
Zn2+ | the sensor domain of CzcS senses the Zn(II) stimulus at micromole level in a tetrahedral geometry through its symmetry-related residues His55 and Asp60 on the dimer interface | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I0V9 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9I0V9 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CzcS | - |
Pseudomonas aeruginosa |
PA2524 | - |
Pseudomonas aeruginosa |
sensor protein czcS | - |
Pseudomonas aeruginosa |