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Literature summary for 2.7.13.3 extracted from

  • Albanesi, D.; Martin, M.; Trajtenberg, F.; Mansilla, M.C.; Haouz, A.; Alzari, P.M.; de Mendoza, D.; Buschiazzo, A.
    Structural plasticity and catalysis regulation of a thermosensor histidine kinase (2009), Proc. Natl. Acad. Sci. USA, 106, 16185-16190.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
the phosphorylated cytoplasmic domain of DesK is crystallized by hanging drop vapor diffusion, using 10% (w/v) PEG 3000, 0.1 M CHES, pH 9.5, and 10 mM MgCl2, at 18°C Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
H188V the mutant retains the phosphatase activity of the wild type protein, the mutation triggers formation of an N-terminal 2-helical coiled-coil and extensive dimerization and histidine phosphotransfer domain-ATP-binding domain association Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protein L-histidine Bacillus subtilis
-
ADP + protein N-phospho-L-histidine
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34757
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
Bacillus subtilis

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography and Superdex 75 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein L-histidine
-
Bacillus subtilis ADP + protein N-phospho-L-histidine
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Bacillus subtilis

Synonyms

Synonyms Comment Organism
DesK
-
Bacillus subtilis
thermosensor histidine kinase
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis

Expression

Organism Comment Expression
Bacillus subtilis DesK is cold-activated up