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Literature summary for 2.7.13.3 extracted from

  • Rasmussen, A.A.; Wegener-Feldbruegge, S.; Porter, S.L.; Armitage, J.P.; Sogaard-Andersen, L.
    Four signalling domains in the hybrid histidine protein kinase RodK of Myxococcus xanthus are required for activity (2006), Mol. Microbiol., 60, 525-534.
    View publication on PubMed
Search for more literature for 2.7.13.3

Application

Application Comment Organism
additional information hybrid histidine protein kinase in which four signalling domains are required for full activity Myxococcus xanthus

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants introduced into deltarodK strain SA1708, His6-tagged wild-type and mutants expressed in Escherichia coli Myxococcus xanthus

Protein Variants

Protein Variants Comment Organism
D657N substitution in the first receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, rare sporulation outside fruiting bodies, but normal fruiting body morphology on CF agar, loose aggregates of fruiting bodies in submerged culutre Myxococcus xanthus
D657N/D782N delayed timing of aggregation, frequent sporulation outside fruiting bodies, and loose mounds of fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, no autophosphorylation Myxococcus xanthus
D657N/D782N/D909N delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates Myxococcus xanthus
D657N/D909N delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular and small fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates Myxococcus xanthus
D782N substitution in the second receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular and small fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre Myxococcus xanthus
D782N/D909N delayed timing of aggregation, frequent sporulation outside fruiting bodies, and loose mounds of fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates Myxococcus xanthus
D909N substitution in the third receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, but normal fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre Myxococcus xanthus
H360A is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre Myxococcus xanthus
additional information delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ATP mutant D657N/D782N/D909N Myxococcus xanthus

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein L-histidine
-
 Myxococcus xanthus ADP + protein N-phospho-L-histidine
-
 ?
additional information no autophosphorylation of wild-type, is caused by the rate of dephosphorylation being higher than the rate of autophosphorylation Myxococcus xanthus ?
-
 ?

Synonyms

Synonyms Comment Organism
histidine protein kinase
-
 Myxococcus xanthus
RodK
-
 Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000167
-
 ATP mutant D657N/D782N/D909N Myxococcus xanthus