Literature summary for 2.7.13.3 extracted from

Yamada, S.; Akiyama, S.; Sugimoto, H.; Kumita, H.; Ito, K.; Fujisawa, T.; Nakamura, H.; Shiro, Y.
The signaling pathway in histidine kinase and the response regulator complex revealed by X-ray crystallography and solution scattering (2006), J. Mol. Biol., 362, 123-139.

Search for more literature for 2.7.13.3

Application

Application	Comment	Organism
additional information	responsible role of the response regulator TrrA for the feedback regulation of sensing and/or kinase activities of ThkA	Thermotoga maritima

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) CodonPlus RIL-X	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
ThkA complexed with the response regulator TrrA, by the batch method, X-ray crystallography at a resolution of 4.2 A, 2fold symmetry, two monomeric TrrAs bind to the ThkA dimer	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
additional information	delta408ThkA, includes the PAS-sensor, the DHp and CA domains, and delta 517ThkA without the PAS-sensor domain, both are fully active in the autophosphorylation reaction	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51000	-	delta517ThkA, experimental estimates	Thermotoga maritima
76000	-	delta408ThkA, experimental estimates	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9X180	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	autophosphorylation activity	Thermotoga maritima	?	-	?

Subunits

Subunits	Comment	Organism
dimer	crystallography	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
histidine kinase	-	Thermotoga maritima
ThkA	-	Thermotoga maritima

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)