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Literature summary for 2.7.13.3 extracted from
- Osmosensing properties of the histidine protein kinase MtrB from Corynebacterium glutamicum (2007), J. Biol. Chem., 282, 27666-27677.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alanine | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum |  |
| betaine | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| fructose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| glucose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| glutamate | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| L-glutamate | stimulates, more efficient stimulation of MtrB than MtrA | Corynebacterium glutamicum | |
| lysine | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| maltoheptaose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| maltose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| maltotriose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| additional information | liposome shrinkage is not necessary for MtrB activation, MtrB is activated upon the addition of various chemical compounds, like sugars, amino acids, and polyethylene glycols, these may act via a change of the hydration state of the protein shifting MtrB into the active state, for activation it is essential that solutes are added at the same side as the cytoplasmic domains of the kinase are located | Corynebacterium glutamicum | |
| NaCl | provides minor activation | Corynebacterium glutamicum | |
| proline | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| sucrose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
| trehalose | stimulates, stimulation pattern of MtrA is similar to that of MtrB | Corynebacterium glutamicum | |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | MtrB belongs to a class of histidine protein kinases that sense environmental changes at cytoplasmatic protein domains independently of the periplasmic loop and the cytoplasmic HAMP domain | Corynebacterium glutamicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli BL21(DE3) cells transformed with different pASK-IBA7-mtrB strep derivatives | Corynebacterium glutamicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | in the deltamtrAB mutant, the expression of carrier genes is abolished (betP) or markedly reduced, MtrB mutants in which either the large periplasmic loop or the HAMP domain is deleted, are regulated similar to wild-type MtrB | Corynebacterium glutamicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Corynebacterium glutamicum | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE of MtrB | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
strain ATCC 13032 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Corynebacterium glutamicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 50 mM Tris/HCl buffer, pH 8.0, 500 mM NaCl, 10% glycerol, 10 mM beta-mercaptoethanol | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | autophosphorylation of MtrB-Strep in proteoliposomes in the presence of ATP | Corynebacterium glutamicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE of MtrB | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| histidine protein kinase | - |
Corynebacterium glutamicum |
| MtrA | - |
Corynebacterium glutamicum |
| MtrB | - |
Corynebacterium glutamicum |
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