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Literature summary for 2.7.13.3 extracted from
- Temperature and urea induced conformational changes of the histidine kinases from Mycobacterium tuberculosis (2007), Int. J. Biol. Macromol., 41, 154-161.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | great effect of temperature on the dynamics of the HAMP linker domain, which is a key feature in signal transduction | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pQE30 and overexpressed in Escherichia coli M15 | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | thermal transition of HK1 is a two-state process and that of HK2 is a three-state process. Urea denaturation of HK1 and HK2 is a three-state and two-state process, respectively | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-NTA column and gel filtration | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| histidine kinase | - |
Mycobacterium tuberculosis |
| HK1 | - |
Mycobacterium tuberculosis |
| HK2 | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
HK2 is stable up to 40°C and reaches an intermediate state at 60°C. On raising the temperature to 100°C, the second stage of denaturation is observed | Mycobacterium tuberculosis |
| 60 | - |
HK1 relatively stable up to 60°C, gets completely denatured at 90°C | Mycobacterium tuberculosis |
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