Cloned (Comment) | Organism |
---|---|
gene bos5 | Botrytis cinerea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a bos5 gene deletion mutant DELTAbcos5-21 by gene replacement using Agrobacterium tumefaciens strain C58C1, complementation of the bos5 deletion mutant DELTAbos5-21 pathogenicity test in cucumber leaves, the DELTAbos5-21 is unable to infect cucumber leaves at all overview | Botrytis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Botrytis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BcSak1 | Botrytis cinerea | - |
ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | Botrytis cinerea 38B1 | - |
ADP + phosphorylated BcSak1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botrytis cinerea | I7FWY1 | i.e. Botryotinia fuckeliana | - |
Botrytis cinerea 38B1 | I7FWY1 | i.e. Botryotinia fuckeliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BcSak1 | - |
Botrytis cinerea | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | protei BcSAK1 is the putative downstream component of BOS5 | Botrytis cinerea | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | - |
Botrytis cinerea 38B1 | ADP + phosphorylated BcSak1 | - |
? | |
ATP + BcSak1 | protei BcSAK1 is the putative downstream component of BOS5 | Botrytis cinerea 38B1 | ADP + phosphorylated BcSak1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BcOs4 | - |
Botrytis cinerea |
BOS5 | - |
Botrytis cinerea |
MAPKK | - |
Botrytis cinerea |
mitogen-activated protein kinase kinase | - |
Botrytis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Botrytis cinerea |
General Information | Comment | Organism |
---|---|---|
evolution | bos5 from Botrytis cinerea, a gene that encodes a mitogen-activated protein kinase kinase (MAPKK), is homologous to OS-5 of Neurospora crassa | Botrytis cinerea |
malfunction | the bos5 gene deletion mutant exhibits reduced vegetative growth and strongly impaired conidiation. The mutant also exhibits increased sensitivity to the dicarboximide fungicide iprodione and to osmotic stress mediated by NaCl or KCl. The BcSAK1 protein, the putative downstream component of BOS5, is not phosphorylated in the mutant. Plant inoculation tests show that the mutants are unable to infect cucumber leaves. All of these defects are restored by genetic complementation of the DELTAbcos5-21 mutant with the wild-type bos5 gene | Botrytis cinerea |
physiological function | the mitogen-activated protein kinase kinase BOS5 is involved in regulating vegetative differentiation and virulence in Botrytis cinerea | Botrytis cinerea |