Literature summary for 2.7.12.2 extracted from

Matsumoto, T.; Kinoshita, T.; Kirii, Y.; Tada, T.; Yamano, A.
Crystal and solution structures disclose a putative transient state of mitogen-activated protein kinase kinase 4 (2012), Biochem. Biophys. Res. Commun., 425, 195-200.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant apo MAP2K4-KD or in complex with ANP, 15 mg/ml protein in a solution containing 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10% glycerol and 10 mM DTT for the apoenzyme, and 2 mM ANP and MgCl2 additional for the npMAP2K4/ANP complex, mixing with reservoir solution containing 0.2 M ammonium acetate, 22-25% w/v PEG 3350 and 0.1 M HEPESNaOH, pH 7.0, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant apo MAP2K4-KD or in complex with ANP, 15 mg/ml protein in a solution containing 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10% glycerol and 10 mM DTT for the apoenzyme, and 2 mM ANP and MgCl2 additional for the npMAP2K4/ANP complex, mixing with reservoir solution containing 0.2 M ammonium acetate, 22-25% w/v PEG 3350 and 0.1 M HEPESNaOH, pH 7.0, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution

Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37950	-	-	Homo sapiens
39000	-	-	Homo sapiens
40000	-	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P45985	-	-

Subunits

Subunits	Comment	Organism
?	x * 37419 , apo npMAP2K4, sequence calculation, x * 37950, npMAP2K4/ANP complex, sequence calculation, x * 39000 , apo npMAP2K4, light scattering, x * 40000, npMAP2K4/ANP complex, light scattering	Homo sapiens

Synonyms

Synonyms	Comment	Organism
MAP2K4	-	Homo sapiens
mitogen-activated protein kinase kinase 4	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	upon ATP-binding, the transient conformation adopted the configuration of typical kinase folding. In the absence of ATP-binding, the transient state of apo npMAP2K4 may shift to a state of aggregation via non-particular hydrophobic interactions as a result of the exposed hydrophobic residues	Homo sapiens

General Information

General Information	Comment	Organism
additional information	the transient state of apo npMAP2K4 exists between the canonical kinase fold and the aggregation state. The ATP molecule under physiological conditions allows the transient conformation to rapidly assume the canonical kinase fold but the depletion of ATP under conditions of low levels of bioactivity perhaps enhances the transition to the aggregation state	Homo sapiens
physiological function	mitogen-activated protein kinase kinase 4 (MAP2K4) plays a crucial role in the stress-activated signal cascade and is enzymatically regulated by ligand or substrate binding, and/or post-translational modification	Homo sapiens