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Literature summary for 2.7.12.1 extracted from

  • Zhou, N.; Yuan, S.; Wang, R.; Zhang, W.; Chen, J.J.
    Role of dual specificity tyrosine-phosphorylation-regulated kinase 1B (Dyrk1B) in S-phase entry of HPV E7 expressing cells from quiescence (2015), Oncotarget, 6, 30745-30761.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.12.1

Application

Application Comment Organism
medicine Dyrk1B may serve as a target for therapy in human papillomavirus-associated cancers Homo sapiens

Protein Variants

Protein Variants Comment Organism
T198A site-directed mutagenesis, the mutation reduces the enzyme activity compared to the wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus the majority of Dyrk1B proteins are localized in the nucleus in both vector and E7 expressing cells Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + p27 Homo sapiens p27 phosphorylation at Ser10 and Thr198 ADP + phospho-p27
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from both vector and E7 expressing cells by subcellular fractionation Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
RPE-1 cell retinal pigmented epithelial cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + p27 p27 phosphorylation at Ser10 and Thr198 Homo sapiens ADP + phospho-p27
-
 ?
ATP + VSNGSPSLER a p27-derived peptide, residues 6-15 Homo sapiens ADP + VSNGpSPSLER
-
 ?

Synonyms

Synonyms Comment Organism
dual specificity tyrosine-phosphorylation-regulated kinase 1B
-
 Homo sapiens
DYRK1B
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the enzyme is upregulated in cells expressing the high-risk human papillomavirus E7 oncogene, which promotes S-phase entry of host cells from quiescent state in the presence of elevated cell cycle inhibitor p27Kip1 up

General Information

General Information Comment Organism
malfunction knockdown of Dyrk1B by siRNA leads to a modest but statistically significant reduction of BrdU incorporation in RPE1-E7 cells, and reduction of Dyrk1B or mutation T198A significantly reduce S10P in E7 expressing cells Homo sapiens
physiological function enzyme Dyrk1B is a dual-specificity kinase and negative regulator of cell proliferation in quiescent cells. Dyrk1B plays a positive role in S-phase entry of quiescent high-risk human papillomavirus E7 oncogene, HPV E7, expressing cells, and Dyrk1B upregulates HPV E7. Dyrk1B contributes to p27 phosphorylation (at Ser10 and Thr198), which is important for the proliferation of HPV E7 expressing cells. DYRK1B overexpression does not increase S-phase entry in control RPE1 cells Homo sapiens