Literature summary for 2.7.12.1 extracted from

Lerner-Marmarosh, N.; Shen, J.; Torno, M.D.; Kravets, A.; Hu, Z.; Maines, M.D.
Human biliverdin reductase: A member of the insulin receptor substrate family with serine/threonine/tyrosine kinase activity (2005), Proc. Natl. Acad. Sci. USA, 102, 7109-7114.

Search for more literature for 2.7.12.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain DH5alpha as GST-fusion protein, expression in 293A cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme knock-out leads to increased D-glucose uptake in response to insulin	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
genistein	-	Homo sapiens
Zn2+	inhibits the autophosphoylation, which cannot be reversed by Mn2+ addition	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for kinase activity	Homo sapiens
Mn2+	essential for autophosphorylation activity, cannot be substituted by Mg2+, Ca2+, or Zn2+	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + insulin receptor kinase substrate 1	Homo sapiens	phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway	ADP + phosphorylated insulin receptor kinase substrate 1	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzyme autophosphorylates at Thr202, Tyr72 and Tyr83, the insulin-activated insulin receptor kinase phosphorylates Y198 in the YMKM motif, Y228 in the YLSF motif, and Y291	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-fusion protein from Escherichia coli strain DH5alpha by glutathione affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + casein	-	Homo sapiens	ADP + phosphorylated casein	-	?
ATP + insulin receptor kinase substrate 1	phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway	Homo sapiens	ADP + phosphorylated insulin receptor kinase substrate 1	-	?
ATP + insulin receptor kinase substrate 1	phosphorylation at serine residues leading to blockage of insulin action, overview	Homo sapiens	ADP + phosphorylated insulin receptor kinase substrate 1	-	?
ATP + myelin basic protein	-	Homo sapiens	ADP + phosphorylated myelin basic protein	-	?
additional information	the enzyme performs autophosphorylation at Thr202, Tyr72 and Tyr83, the enzyme also shows biliverdin reductase activity	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
biliverdin reductase	-	Homo sapiens
BVR	-	Homo sapiens
More	the enzyme belongs to the insulin receptor substrate family	Homo sapiens
serine/threonine/tyrosine kinase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)