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Literature summary for 2.7.12.1 extracted from

  • Rudrabhatla, P.; Rajasekharan, R.
    Functional characterization of peanut serine/threonine/tyrosine protein kinase: molecular docking and inhibition kinetics with tyrosine kinase inhibitors (2004), Biochemistry, 43, 12123-12132.
    View publication on PubMed
Search for more literature for 2.7.12.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Arachis hypogaea

Protein Variants

Protein Variants Comment Organism
K160R site-directed mutagenesis, inactive mutant enzyme Arachis hypogaea
Y148F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea
Y213F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea
Y297F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea
Y317F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
genistein competitive inhibition versus ATP, uncompetitive inhibition versus histone H1 Arachis hypogaea
additional information molecular docking with tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes Arachis hypogaea
staurosporine competitive versus ATP, mixed inhibition versus histone H1 Arachis hypogaea
tyrphostin competitive versus ATP, mixed inhibition versus histone H1 Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Arachis hypogaea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 recombinant His-tagged wild-type enzyme, gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arachis hypogaea the enzyme is developmentally regulated ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme performs autophosphorylation, mechanism and phosphorylation sites, overview Arachis hypogaea

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H1
-
 Arachis hypogaea ADP + phosphorylated histone H1
-
 ?
additional information the enzyme is developmentally regulated Arachis hypogaea ?
-
 ?

Subunits

Subunits Comment Organism
More structure modeling of wild-type and mutant enzymes based on the X-ray structure of homologous sequences, computational docking and molecular dynamics simulations, overview Arachis hypogaea

Synonyms

Synonyms Comment Organism
serine/threonine/tyrosine protein kinase
-
 Arachis hypogaea
STY protein kinase
-
 Arachis hypogaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arachis hypogaea

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arachis hypogaea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics of tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes Arachis hypogaea
0.000014
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
0.000017
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
0.000018
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
0.00002
-
 genistein versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
0.000055
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
0.000056
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
0.000059
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
0.000069
-
 staurosporine versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
0.000112
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
0.000186
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea
0.000187
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
0.000191
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
0.000194
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea
0.000202
-
 tyrphostin versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
0.000426
-
 genistein versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
0.000455
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
0.000464
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea
0.000549
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
0.000571
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
0.000599
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
0.000621
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
0.000694
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea