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Literature summary for 2.7.11.7 extracted from

  • Mondal, S.; Bakthavatsalam, D.; Steimle, P.; Gassen, B.; Rivero, F.; Noegel, A.A.
    Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions (2008), J. Cell Biol., 181, 747-760.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.7

Activating Compound

Activating Compound Comment Organism Structure
additional information association with the actin cytoskeleton causes a drastic increase in the autophosphorylation activity of MHCK A, which is then activated Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment) Organism
cells overexpressing GFP-GEF have both unphosphorylated and autophosphorylated MHCK A Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information overexpression of the RasGEF Q GEF domain activates RasB, causes enhanced recruitment of MHCK A to the cortex, and leads to cytokinesis defects in suspension phenocopying cells expressing constitutively active RasB, and myosin-null mutants, phenotype, overview. Cells overexpressing the GEF domain show at least 2fold higher levels of MHCK A associated with the cytoskeletal fractions Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoskeleton MHCK A associates with the cytoskeleton and localizes to the cell cortex Dictyostelium discoideum 5856
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 x * 145000, phosphorylated MHCK A, SDS-PAGE, x * 130000, unphosphorylated MHCK A, SDS-PAGE Dictyostelium discoideum
145000
-
 x * 145000, phosphorylated MHCK A, SDS-PAGE, x * 130000, unphosphorylated MHCK A, SDS-PAGE Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin heavy-chain Dictyostelium discoideum role of RasGEF Q in regulating myosin II function through MHCK A, overview ADP + myosin heavy chain phosphate
-
 ?
additional information Dictyostelium discoideum MHCK A contains active RasB, and is the predominant exchange factor for RasB. RasGEF Q can bind to F-actin and has the potential to form complexes with MHCK A, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum P42527
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein association with the actin cytoskeleton causes a drastic increase in the autophosphorylation activity of MHCK A, which is then activated Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myosin heavy-chain role of RasGEF Q in regulating myosin II function through MHCK A, overview Dictyostelium discoideum ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy-chain phosphorylation of myosin takes place at three threonine residues in the tail region by myosin II heavy chain kinases Dictyostelium discoideum ADP + myosin heavy chain phosphate
-
 ?
additional information MHCK A contains active RasB, and is the predominant exchange factor for RasB. RasGEF Q can bind to F-actin and has the potential to form complexes with MHCK A, overview Dictyostelium discoideum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 145000, phosphorylated MHCK A, SDS-PAGE, x * 130000, unphosphorylated MHCK A, SDS-PAGE Dictyostelium discoideum

Synonyms

Synonyms Comment Organism
MHCK A
-
 Dictyostelium discoideum
myosin heavy chain kinase A
-
 Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum