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Literature summary for 2.7.11.7 extracted from
- Determinants for substrate phosphorylation by Dictyostelium myosin II heavy chain kinases A and B and eukaryotic elongation factor-2 kinase (2008), Biochim. Biophys. Acta, 1784, 908-915.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | - |
Dictyostelium discoideum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of MHCK A, comprising residues 527-887, i.e. A-CAT, as a GST-fusion protein, phylogenetic analysis | Dictyostelium discoideum |
| expression of MHCK B, comprising residues 13-459, i.e. B-CAT, as a GST-fusion protein, phylogenetic analysis | Dictyostelium discoideum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Dictyostelium discoideum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Dictyostelium discoideum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin heavy-chain | Dictyostelium discoideum | - |
ADP + myosin heavy chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | P42527 | MHCK A | - |
| Dictyostelium discoideum | P90648 | MHCK B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant MHCK A GST-tagged catalytic domain by glutathione affinity chromatography | Dictyostelium discoideum |
| recombinant MHCK B GST-tagged catalytic domain by glutathione affinity chromatography | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin heavy-chain | - |
Dictyostelium discoideum | ADP + myosin heavy chain phosphate | - |
? | |
| additional information | substrate specificity of the MHCK A catalytic domain: a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by MHCK A. Phosphorylation by MHCK A is enhanced 2 to 4fold by basic residues in the P+2, P+3 and P+4 positions, with a strong preference for threonine as the phosphoacceptor amino acid, overview | Dictyostelium discoideum | ? | - |
? | |
| additional information | substrate specificity of the MHCK B catalytic domain: a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by MHCK B. Phosphorylation by MHCK B is further enhanced 8fold by a basic residue in the P+2 position, strong preference for threonine as the phosphoacceptor amino acid, overview | Dictyostelium discoideum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MHCK A | - |
Dictyostelium discoideum |
| MHCK B | - |
Dictyostelium discoideum |
| myosin II heavy chain kinases A | - |
Dictyostelium discoideum |
| myosin II heavy chain kinases B | - |
Dictyostelium discoideum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Dictyostelium discoideum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Dictyostelium discoideum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Dictyostelium discoideum | |
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