Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acidic phospholipids | enhance the activation of the enzyme by autophosphorylation | Acanthamoeba castellanii | |
additional information | enzyme is stimulated by its autophosphorylation | Acanthamoeba castellanii | |
phosphatidylserine | - |
Acanthamoeba castellanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
myosin IB | soluble, unphosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.0003 | - |
myosin IA | soluble, unphosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.0005 | - |
myosin IB | phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.0007 | - |
myosin IA | phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.0023 | - |
myosin IA | soluble, phosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.0055 | - |
myosin IB | soluble, phosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.064 | - |
GRGRSSVYS | phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.064 | - |
GRGRSSVYS | soluble, phosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii | |
0.11 | - |
GRGRSSVYS | soluble, unphosphorylated enzyme, pH 7.0, 30°C | Acanthamoeba castellanii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | binding to the plasma membrane enhances the activity of unphosphorylated enzyme 20fold, substrate myosin I | Acanthamoeba castellanii | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acanthamoeba castellanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | - |
myosin I heavy chain kinase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GRGRSSVYS | - |
Acanthamoeba castellanii | ADP + GRGRSS(-phosphate)VYS | - |
? | |
ATP + myosin heavy chain kinase | intermolecular autophosphorylation with vesicle-bound enzyme in absence of substrates | Acanthamoeba castellanii | ADP + myosin heavy chain kinase phosphate | - |
? | |
ATP + myosin heavy chain kinase | leads to activation of the phosphorylation activity towards myosin I | Acanthamoeba castellanii | ADP + myosin heavy chain kinase phosphate | - |
? | |
ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | higher activity with membrane-bound substrate myosin I | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrates are heavy chains of myosin IA and IB | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin IA | - |
Acanthamoeba castellanii | ADP + myosin IA phosphate | - |
? | |
ATP + myosin IB | - |
Acanthamoeba castellanii | ADP + myosin IB phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MHCK | - |
Acanthamoeba castellanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acanthamoeba castellanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acanthamoeba castellanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acanthamoeba castellanii |