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Literature summary for 2.7.11.7 extracted from

  • Rieker, J.P.; Swanljung-Collins, H.; Montibeller, J.; Collins, J.H.
    Isolation and characterization of calmodulin-dependent myosin heavy chain kinase from intestinal brush border (1987), Methods Enzymol., 139, 105-114.
    View publication on PubMed
Search for more literature for 2.7.11.7

General Stability

General Stability Organism
freeze-thawing inactivates Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
EGTA
-
 Gallus gallus
NaCl above 0.1 M Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Gallus gallus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement, Ca2+/calmodulin-dependent isozyme Gallus gallus
Mg2+ requirement Gallus gallus
Mg2+ optimal activation at 6 mM Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin heavy chain Gallus gallus
-
 ADP + myosin heavy chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 two myosin heavy-chain kinases: a Ca2+/calmodulin dependent and a Ca2+/calmodulin independent enzyme
-

Purification (Commentary)

Purification (Comment) Organism
separation of the Ca2+/calmodulin-dependent and -independent isozymes Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
epithelium of the intestine, brush border cells Gallus gallus
-
small intestine epithelium Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00032
-
 partially purified enzyme Gallus gallus

Storage Stability

Storage Stability Organism
0°C, further purified Ca2+/calmodulin-dependent isozyme, complete loss of activity within 1 day Gallus gallus
0°C, partly purified Ca2+/calmodulin-dependent isozyme, at least 2 weeks Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myosin heavy chain
-
 Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain phosphorylates threonine residues Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain best substrate: chicken intestine brush border myosin Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain specific for myosin heavy chains, no phosphorylation of light chains Gallus gallus ADP + myosin heavy chain phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Gallus gallus