Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by calmodulin, cAMP | Acanthamoeba castellanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | no inhibition | Acanthamoeba castellanii | |
additional information | strong inhibition of phosphorylation activity by increasing ionic strength | Acanthamoeba castellanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
ATP | pH 7.5, 30°C | Acanthamoeba castellanii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Acanthamoeba castellanii | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not required | Acanthamoeba castellanii | |
Mg2+ | requirement | Acanthamoeba castellanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
gel filtration | Acanthamoeba castellanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin II heavy chain | Acanthamoeba castellanii | - |
ADP + myosin II heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | - |
myosin I heavy chain kinase | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography using histone-resin | Acanthamoeba castellanii |
to near homogeneity | Acanthamoeba castellanii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Acanthamoeba castellanii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.03 | - |
purified enzyme, substrate histone 2A, 0.067 mM | Acanthamoeba castellanii |
4.6 | - |
purified enzyme, substrate myosin IB, 0.0024 mM | Acanthamoeba castellanii |
Storage Stability | Organism |
---|---|
-20°C, in 50% glycerol, 3 months stable and less than 20% loss of activity within 6 months | Acanthamoeba castellanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | poor substrate | Acanthamoeba castellanii | ADP + casein phosphate | - |
? | |
ATP + histone 2A | - |
Acanthamoeba castellanii | ADP + histone 2A phosphate | - |
? | |
ATP + myosin heavy chain kinase | intramolecular autophosphorylation | Acanthamoeba castellanii | ADP + myosin heavy chain kinase phosphate | - |
? | |
ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate: myosin IB, phosphorylation site is Ser315 | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | phosphorylates serine-residues | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | incorporation of 0.9-1.0 mol phosphate per mol of heavy chain myosin | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | contains two myosin heavy chain kinases: one for myosin I and one for myosin II | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrates are heavy chains of myosin IA and IB | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin II heavy chain | - |
Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
ATP + myosin II heavy chain | contains two myosin heavy chain kinases: one for myosin I and one for myosin II | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
ATP + myosin II heavy chain | phosphorylates serine residues | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
ATP + phosvitin | no activity | Acanthamoeba castellanii | ADP + phosvitin phosphate | - |
? | |
ATP + smooth muscle myosin light chain | - |
Acanthamoeba castellanii | ADP + smooth muscle myosin light chain phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 107000, phosphorylated enzyme, SDS-PAGE | Acanthamoeba castellanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acanthamoeba castellanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Acanthamoeba castellanii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | about half-maximal activity at pH 6.0 | Acanthamoeba castellanii |
6 | 8.5 | about 80% activity at pH 6.0 | Acanthamoeba castellanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acanthamoeba castellanii |