Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by cGMP | Dictyostelium discoideum | |
additional information | no activation by EGTA | Dictyostelium discoideum | |
additional information | no activation by calmodulin, cAMP | Dictyostelium discoideum |
General Stability | Organism |
---|---|
very unstable if exposed to low salt, i.e. 50 mM KCl or less, in the absence of sucrose | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | no inhibition | Dictyostelium discoideum | |
EGTA | no inhibition | Dictyostelium discoideum | |
KCl | strong, 90% inhibition at 60 mM, complete inhibition at 100 mM | Dictyostelium discoideum | |
additional information | no inhibition by Ca2+; no inhibition by cAMP or cGMP | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Dictyostelium discoideum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not required | Dictyostelium discoideum | |
Mg2+ | requirement | Dictyostelium discoideum | |
Mg2+ | absolutely requires 1-2 mM Mg2+ | Dictyostelium discoideum | |
Mg2+ | cannot be substituted by Ca2+ or Mn2+ | Dictyostelium discoideum | |
additional information | no activation by Mn2+ | Dictyostelium discoideum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
x * 130000, SDS-PAGE | Dictyostelium discoideum |
700000 | - |
above, gel filtration | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin I heavy chain | Dictyostelium discoideum | reaction in regulatory contractile activity in Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
14000fold to near homogeneity | Dictyostelium discoideum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | growth-phase cells | Dictyostelium discoideum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.98 | - |
purified enzyme | Dictyostelium discoideum |
Storage Stability | Organism |
---|---|
0°C, in 10 mM imidazole, pH 7, 100 mM KCl, 2 mM DTT, 60% sucrose, several weeks | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | no activity | Dictyostelium discoideum | ADP + casein phosphate | - |
? | |
ATP + histone 2A | low activity | Dictyostelium discoideum | ADP + histone 2A phosphate | - |
? | |
ATP + myosin heavy chain kinase | intramolecular autophosphorylation | Dictyostelium discoideum | ADP + myosin heavy chain kinase phosphate | - |
? | |
ATP + myosin heavy chain kinase | leads to activation of the phosphorylation activity towards myosin I | Dictyostelium discoideum | ADP + myosin heavy chain kinase phosphate | - |
? | |
ATP + myosin I heavy chain | myosin light chains are no substrates | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | phosphorylates threonine residues | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | 1.9 mol phosphate per mol myosin | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | no substrates are human platelet myosin, ovalbumin and smooth muscle myosin from turkey | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | reaction in regulatory contractile activity in Dictyostelium discoideum | Dictyostelium discoideum | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + phosvitin | low activity | Dictyostelium discoideum | ADP + phosvitin phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, SDS-PAGE | Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Dictyostelium discoideum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | about half-maximal activity at pH 6.0 | Dictyostelium discoideum |
6 | 8.5 | about 50% activity at pH 6.0 | Dictyostelium discoideum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
and above, unstable | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dictyostelium discoideum |