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Literature summary for 2.7.11.7 extracted from

  • Côté, G.P.; Bukiejko, U.
    Purification and characterization of a myosin heavy chain kinase from Dictyostelium discoideum (1987), J. Biol. Chem., 262, 1065-1072.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by cGMP Dictyostelium discoideum
additional information no activation by EGTA Dictyostelium discoideum
additional information no activation by calmodulin, cAMP Dictyostelium discoideum

General Stability

General Stability Organism
very unstable if exposed to low salt, i.e. 50 mM KCl or less, in the absence of sucrose Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+/calmodulin no inhibition Dictyostelium discoideum
EGTA no inhibition Dictyostelium discoideum
KCl strong, 90% inhibition at 60 mM, complete inhibition at 100 mM Dictyostelium discoideum
additional information no inhibition by Ca2+; no inhibition by cAMP or cGMP Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Dictyostelium discoideum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not required Dictyostelium discoideum
Mg2+ requirement Dictyostelium discoideum
Mg2+ absolutely requires 1-2 mM Mg2+ Dictyostelium discoideum
Mg2+ cannot be substituted by Ca2+ or Mn2+ Dictyostelium discoideum
additional information no activation by Mn2+ Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
x * 130000, SDS-PAGE Dictyostelium discoideum
700000
-
above, gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin I heavy chain Dictyostelium discoideum reaction in regulatory contractile activity in Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
14000fold to near homogeneity Dictyostelium discoideum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture growth-phase cells Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.98
-
purified enzyme Dictyostelium discoideum

Storage Stability

Storage Stability Organism
0°C, in 10 mM imidazole, pH 7, 100 mM KCl, 2 mM DTT, 60% sucrose, several weeks Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein no activity Dictyostelium discoideum ADP + casein phosphate
-
?
ATP + histone 2A low activity Dictyostelium discoideum ADP + histone 2A phosphate
-
?
ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
?
ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
?
ATP + myosin I heavy chain myosin light chains are no substrates Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain 1.9 mol phosphate per mol myosin Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain no substrates are human platelet myosin, ovalbumin and smooth muscle myosin from turkey Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain reaction in regulatory contractile activity in Dictyostelium discoideum Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
?
ATP + phosvitin low activity Dictyostelium discoideum ADP + phosvitin phosphate
-
?

Subunits

Subunits Comment Organism
? x * 130000, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Dictyostelium discoideum

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 about half-maximal activity at pH 6.0 Dictyostelium discoideum
6 8.5 about 50% activity at pH 6.0 Dictyostelium discoideum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
and above, unstable Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Dictyostelium discoideum