BRENDA - Enzyme Database show
show all sequences of 2.7.11.32

Posttranslational modification of maize chloroplast pyruvate orthophosphate dikinase reveals the precise regulatory mechanism of its enzymatic activity

Chen, Y.B.; Lu, T.C.; Wang, H.X.; Shen, J.; Bu, T.T.; Chao, Q.; Gao, Z.F.; Zhu, X.G.; Wang, Y.F.; Wang, B.C.; Plant Physiol. 165, 534-549 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Zea mays
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + [pyruvate, phosphate dikinase]
Zea mays
reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK
AMP + [pyruvate, phosphate dikinase] phosphate
-
-
r
additional information
Zea mays
PPDK regulatory protein (PDRP) is a unique bifunctional enzyme, catalyzes this light-dependent regulation by reversible phosphorylation of an active-site Thr527 in PPDK
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Zea mays
-
mesophyll
-
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + [pyruvate, phosphate dikinase]
reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK
739352
Zea mays
AMP + [pyruvate, phosphate dikinase] phosphate
-
-
-
r
ADP + [pyruvate, phosphate dikinase]
i.e. recombinant His-tagged wild-type and mutant maize PPDKs as substrates, reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK. Phosphorylation is detected on wild-type PPDK, PPDK-T309A, and PPDK-S506A, but not on PPDK-T527A, PPDK-S528A, or PPDK-H529A
739352
Zea mays
AMP + [pyruvate, phosphate dikinase] phosphate
-
-
-
r
additional information
PPDK regulatory protein (PDRP) is a unique bifunctional enzyme, catalyzes this light-dependent regulation by reversible phosphorylation of an active-site Thr527 in PPDK
739352
Zea mays
?
-
-
-
-
additional information
modeling suggests that the two hydrogen bonds between the highly conserved residues Ser528 and Gly525 are required for PDRP-mediated phosphorylation of the active-site Thr527 of PPDK, substrate PPDK three-dimensional structure analysis, overview
739352
Zea mays
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
-
Zea mays
AMP
-
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
-
Zea mays
AMP
-
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Zea mays
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP + [pyruvate, phosphate dikinase]
Zea mays
reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK
AMP + [pyruvate, phosphate dikinase] phosphate
-
-
r
additional information
Zea mays
PPDK regulatory protein (PDRP) is a unique bifunctional enzyme, catalyzes this light-dependent regulation by reversible phosphorylation of an active-site Thr527 in PPDK
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Zea mays
-
mesophyll
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + [pyruvate, phosphate dikinase]
reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK
739352
Zea mays
AMP + [pyruvate, phosphate dikinase] phosphate
-
-
-
r
ADP + [pyruvate, phosphate dikinase]
i.e. recombinant His-tagged wild-type and mutant maize PPDKs as substrates, reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK. Phosphorylation is detected on wild-type PPDK, PPDK-T309A, and PPDK-S506A, but not on PPDK-T527A, PPDK-S528A, or PPDK-H529A
739352
Zea mays
AMP + [pyruvate, phosphate dikinase] phosphate
-
-
-
r
additional information
PPDK regulatory protein (PDRP) is a unique bifunctional enzyme, catalyzes this light-dependent regulation by reversible phosphorylation of an active-site Thr527 in PPDK
739352
Zea mays
?
-
-
-
-
additional information
modeling suggests that the two hydrogen bonds between the highly conserved residues Ser528 and Gly525 are required for PDRP-mediated phosphorylation of the active-site Thr527 of PPDK, substrate PPDK three-dimensional structure analysis, overview
739352
Zea mays
?
-
-
-
-
General Information
General Information
Commentary
Organism
additional information
catalytic mechanism of enzyme PDRP, overview
Zea mays
physiological function
in C4 plants, pyruvate orthophosphate dikinase (PPDK) activity is tightly dark/light regulated by reversible phosphorylation of an active-site threonine residue. The process is catalyzed by PPDK regulatory protein (PDRP). Phosphorylation and dephosphorylation of PPDK lead to its inactivation and activation, respectively. The amount of PPDK (unphosphorylated) involved in C4 photosynthesis is indeed strictly controlled by light intensity, despite the high levels of PPDK protein that accumulate in mesophyll chloroplasts, regulation by light intensity rather than the light/dark transition. Diverse regulatory pathways may work alone or in combination to fine-tune C4PPDK activity in response to changes in lighting. Residue Ser528 plays an essential role in PDRP regulation of PPDK phosphorylation at Thr527
Zea mays
General Information (protein specific)
General Information
Commentary
Organism
additional information
catalytic mechanism of enzyme PDRP, overview
Zea mays
physiological function
in C4 plants, pyruvate orthophosphate dikinase (PPDK) activity is tightly dark/light regulated by reversible phosphorylation of an active-site threonine residue. The process is catalyzed by PPDK regulatory protein (PDRP). Phosphorylation and dephosphorylation of PPDK lead to its inactivation and activation, respectively. The amount of PPDK (unphosphorylated) involved in C4 photosynthesis is indeed strictly controlled by light intensity, despite the high levels of PPDK protein that accumulate in mesophyll chloroplasts, regulation by light intensity rather than the light/dark transition. Diverse regulatory pathways may work alone or in combination to fine-tune C4PPDK activity in response to changes in lighting. Residue Ser528 plays an essential role in PDRP regulation of PPDK phosphorylation at Thr527
Zea mays
Other publictions for EC 2.7.11.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739352
Chen
Posttranslational modification ...
Zea mays
Plant Physiol.
165
534-549
2014
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723426
Astley
The pyruvate, orthophosphate d ...
Arabidopsis thaliana
Plant J.
68
1070-1080
2011
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694671
Chastain
The pyruvate, orthophosphate d ...
Arabidopsis thaliana
Plant J.
53
854-863
2008
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671766
Burnell
Cloning and expression of maiz ...
Zea mays
Biochem. Biophys. Res. Commun.
345
675-680
2006
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676721
Chastain
Posttranslational regulation o ...
Oryza sativa
Planta
224
924-934
2006
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645466
Chastain
-
Regulation of pyruvate, orthop ...
Zea mays
Plant Physiol. Biochem.
41
523-532
2003
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645460
Chastain
Further analysis of maize C(4) ...
Zea mays
Arch. Biochem. Biophys.
375
165-170
2000
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Chastain
Maize recombinant C4-pyruvate, ...
Zea mays
Photosyn. Res.
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1996
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Zea mays
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6683-6687
1988
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Budde
Substrate specificity and regu ...
Zea mays
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1986
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721404
Burnell
Regulation of C4 photosynthesi ...
Zea mays
Arch. Biochem. Biophys.
237
490-503
1985
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3
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721403
Burnell
Regulation of C4 photosynthesi ...
Zea mays
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231
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1984
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