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Literature summary for 2.7.11.32 extracted from

  • Chastain, C.J.; Xu, W.; Parsley, K.; Sarath, G.; Hibberd, J.M.; Chollet, R.
    The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis possess a novel, unprecedented Ser/Thr protein kinase primary structure (2008), Plant J., 53, 854-863.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
cytosol
-
Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49562
-
-
Arabidopsis thaliana Q9MAC9
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information green tissues Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-
seed
-
Arabidopsis thaliana
-
stamen
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate, phosphate dikinase + ADP His458 phosphorylated (active), maize wild type and T456S mutant pyruvate, phosphate dikinase serve as substrate, T456V mutant pyruvate, phosphate dikinase serves not as substrate Arabidopsis thaliana [pyruvate, phosphate dikinase] phosphate + AMP His458 and Thr456 phosphorylated (inactive) ?
pyruvate, phosphate dikinase + ADP His458 phosphorylated, active Arabidopsis thaliana [pyruvate, phosphate dikinase] phosphate + AMP His458 and Thr456 phosphorylated (inactive) ?

Synonyms

Synonyms Comment Organism
AtRP1
-
Arabidopsis thaliana
AtRP2
-
Arabidopsis thaliana
pyruvate, orthophosphate dikinase regulatory protein 1 bifunctional Ser/Thr protein kinase/protein phosphatase Arabidopsis thaliana
pyruvate, orthophosphate dikinase regulatory protein 2 monofunctional Ser/Thr protein kinase Arabidopsis thaliana