BRENDA - Enzyme Database show
show all sequences of 2.7.11.32

The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis possess a novel, unprecedented Ser/Thr protein kinase primary structure

Chastain, C.J.; Xu, W.; Parsley, K.; Sarath, G.; Hibberd, J.M.; Chollet, R.; Plant J. 53, 854-863 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3); His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3)
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
cytosol
-
Arabidopsis thaliana
5829
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O49562
-
-
Arabidopsis thaliana
Q9MAC9
-
-
Purification (Commentary)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+); immobilized metal ion affinity chromatography (Ni2+)
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
green tissues
Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-
seed
-
Arabidopsis thaliana
-
stamen
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate, phosphate dikinase + ADP
His458 phosphorylated (active), maize wild type and T456S mutant pyruvate, phosphate dikinase serve as substrate, T456V mutant pyruvate, phosphate dikinase serves not as substrate
694671
Arabidopsis thaliana
[pyruvate, phosphate dikinase] phosphate + AMP
His458 and Thr456 phosphorylated (inactive)
-
-
?
pyruvate, phosphate dikinase + ADP
His458 phosphorylated, active
694671
Arabidopsis thaliana
[pyruvate, phosphate dikinase] phosphate + AMP
His458 and Thr456 phosphorylated (inactive)
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3)
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
cytosol
-
Arabidopsis thaliana
5829
-
Purification (Commentary) (protein specific)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+)
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
green tissues
Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-
seed
-
Arabidopsis thaliana
-
stamen
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate, phosphate dikinase + ADP
His458 phosphorylated (active), maize wild type and T456S mutant pyruvate, phosphate dikinase serve as substrate, T456V mutant pyruvate, phosphate dikinase serves not as substrate
694671
Arabidopsis thaliana
[pyruvate, phosphate dikinase] phosphate + AMP
His458 and Thr456 phosphorylated (inactive)
-
-
?
pyruvate, phosphate dikinase + ADP
His458 phosphorylated, active
694671
Arabidopsis thaliana
[pyruvate, phosphate dikinase] phosphate + AMP
His458 and Thr456 phosphorylated (inactive)
-
-
?
Other publictions for EC 2.7.11.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739352
Chen
Posttranslational modification ...
Zea mays
Plant Physiol.
165
534-549
2014
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723426
Astley
The pyruvate, orthophosphate d ...
Arabidopsis thaliana
Plant J.
68
1070-1080
2011
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694671
Chastain
The pyruvate, orthophosphate d ...
Arabidopsis thaliana
Plant J.
53
854-863
2008
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5
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2
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671766
Burnell
Cloning and expression of maiz ...
Zea mays
Biochem. Biophys. Res. Commun.
345
675-680
2006
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1
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676721
Chastain
Posttranslational regulation o ...
Oryza sativa
Planta
224
924-934
2006
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645466
Chastain
-
Regulation of pyruvate, orthop ...
Zea mays
Plant Physiol. Biochem.
41
523-532
2003
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1
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2
1
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645460
Chastain
Further analysis of maize C(4) ...
Zea mays
Arch. Biochem. Biophys.
375
165-170
2000
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3
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723347
Chastain
Maize recombinant C4-pyruvate, ...
Zea mays
Photosyn. Res.
49
83-89
1996
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722587
Roeske
Sequence of the phosphothreony ...
Zea mays
J. Biol. Chem.
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1988
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721518
Budde
Substrate specificity and regu ...
Zea mays
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1986
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721404
Burnell
Regulation of C4 photosynthesi ...
Zea mays
Arch. Biochem. Biophys.
237
490-503
1985
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1
3
2
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1
3
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721403
Burnell
Regulation of C4 photosynthesi ...
Zea mays
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1984
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