Cloned (Comment) | Organism |
---|---|
- |
Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | likely inhibits the threonyl-phosphorylation of pyruvate, orthophosphate dikinase by direct competition with regulatory protein for the requisite E-HisP reaction intermediate during catalysis by pyruvate, orthophosphate dikinase, pH not specified in the publication, temperature not specified in the publication | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
pyruvate, phosphate dikinase | pH not specified in the publication, temperature not specified in the publication | Zea mays | |
0.05 | - |
ADP | pH not specified in the publication, temperature not specified in the publication | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Zea mays | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate, phosphate dikinase + ADP | His458 phosphorylated, active | Zea mays | [pyruvate, phosphate dikinase] phosphate + AMP | His458 and Thr456 phosphorylated (inactive), T456S mutant protein substrate is also phosphorylated, H456N mutant protein substrat is not phosphorylated | ? |
Subunits | Comment | Organism |
---|---|---|
More | additional information from earlier studies included (a) estimates of a monomeric molecular mass of 45000-48000, as determined by size exclusion chromatography and one-dimensional SDS-PAGE; (b) pH-dependent changes in aggregation state (dimeric at pH 7.5, tetrameric at pH 8.3) | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
PPDK regulatory protein | - |
Zea mays |
pyruvate, orthophosphate dikinase regulatory protein | bifunctional protein kinase/phosphatase | Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
pyruvate | likely inhibits the threonyl-phosphorylation of pyruvate, orthophosphate dikinase by direct competition with regulatory protein for the requisite E-HisP reaction intermediate during catalysis by pyruvate, orthophosphate dikinase, pH not specified in the publication, temperature not specified in the publication | Zea mays |