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Literature summary for 2.7.11.32 extracted from

  • Chastain, C.J.; Chollet, R.
    Regulation of pyruvate, orthophosphate dikinase by ADP-/Pi-dependent reversible phosphorylation in C3 and C4 plants (2003), Plant Physiol. Biochem., 41, 523-532.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
-
Zea mays

Inhibitors

Inhibitors Comment Organism Structure
pyruvate likely inhibits the threonyl-phosphorylation of pyruvate, orthophosphate dikinase by direct competition with regulatory protein for the requisite E-HisP reaction intermediate during catalysis by pyruvate, orthophosphate dikinase, pH not specified in the publication, temperature not specified in the publication Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
pyruvate, phosphate dikinase pH not specified in the publication, temperature not specified in the publication Zea mays
0.05
-
ADP pH not specified in the publication, temperature not specified in the publication Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Zea mays 9507
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate, phosphate dikinase + ADP His458 phosphorylated, active Zea mays [pyruvate, phosphate dikinase] phosphate + AMP His458 and Thr456 phosphorylated (inactive), T456S mutant protein substrate is also phosphorylated, H456N mutant protein substrat is not phosphorylated ?

Subunits

Subunits Comment Organism
More additional information from earlier studies included (a) estimates of a monomeric molecular mass of 45000-48000, as determined by size exclusion chromatography and one-dimensional SDS-PAGE; (b) pH-dependent changes in aggregation state (dimeric at pH 7.5, tetrameric at pH 8.3) Zea mays

Synonyms

Synonyms Comment Organism
PPDK regulatory protein
-
Zea mays
pyruvate, orthophosphate dikinase regulatory protein bifunctional protein kinase/phosphatase Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.08
-
pyruvate likely inhibits the threonyl-phosphorylation of pyruvate, orthophosphate dikinase by direct competition with regulatory protein for the requisite E-HisP reaction intermediate during catalysis by pyruvate, orthophosphate dikinase, pH not specified in the publication, temperature not specified in the publication Zea mays