Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.31 extracted from

  • Rajamohan, F.; Harris, M.S.; Frisbie, R.K.; Hoth, L.R.; Geoghegan, K.F.; Valentine, J.J.; Reyes, A.R.; Landro, J.A.; Qiu, X.; Kurumbail, R.G.
    Escherichia coli expression, purification and characterization of functional full-length recombinant alpha2beta2gamma3 heterotrimeric complex of human AMP-activated protein kinase (2010), Protein Expr. Purif., 73, 189-197.
    View publication on PubMed
Search for more literature for 2.7.11.31

Activating Compound

Activating Compound Comment Organism Structure
AMP an allosteric activator for AMPK heterotrimeric complex (alpha2beta2gamma3) Homo sapiens
additional information small molecule activator thienopyridone A-769662 has no ativating effect on AMPK heterotrimeric complex (alpha2beta2gamma3) Homo sapiens

Application

Application Comment Organism
drug development AMPK alpha2beta2gamma3 is an attractive target for drug development for diabetes and metabolic syndrome Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
functional His-tagged full-length recombinant alpha2beta2gamma3 heterotrimeric complex of human AMP-activated protein kinase is recombinantly expressed in Escherichia coli strain CodonPlus™(DE3)-RIPL. All three subunits of AMPK alpha2beta2gamma3 are transcribed as a single tricistronic transcript driven by the T7 RNA polymerase promoter, allowing spontaneous formation of the heterotrimeric complex in the bacterial cytosol Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
-
 Homo sapiens
64000
-
-
 Homo sapiens
155000
-
 recombinant His-tagged full-length alpha2beta2gamma3 heterotrimeric AMPK complex, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P54646 AND O43741 AND Q9UGI9 AMPKalpha2, AMPKbeta2, and AMPKgamma3
-

Purification (Commentary)

Purification (Comment) Organism
functional full-length recombinant alpha2beta2gamma3 heterotrimeric complex of human AMP-activated protein kinase from Escherichia coli strain CodonPlus™(DE3)-RIPL by nickel affinity chromatography, dialysis and two steps of gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle in human skeletal muscle, the alpha2beta2gamma3 complexes constitutes the majority of AMPK heterotrimers Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Cy5-SAMS peptide
-
 Homo sapiens ADP + phosphorylated Cy5-SAMS peptide
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 64000, recombinant His-tagged alpha2 subunit, + 1 * 54000, recombinant His-tagged beta2 subunit, + 1* 33000, recombinant His-tagged gamma3 subunit, SDS-PAGE Homo sapiens
More AMPK is a heterotrimeric enzyme with a catalytic (alpha) subunit and two regulatory (beta and gamma) subunits Homo sapiens

Synonyms

Synonyms Comment Organism
AMP-activated protein kinase
-
 Homo sapiens
AMPK
-
 Homo sapiens
AMPK-223
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function AMP-activated protein kinase (AMPK) is an energy-sensing serine/threonine protein kinase that plays a central role in whole-body energy homeostasis. The muscle-specific AMPK heterotrimeric complex (alpha2beta2gamma3) is involved in glucose and fat metabolism in skeletal muscle Homo sapiens