Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation of the enzyme by phosphorylation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged AMPK catalytic domain in Escherichia coli strain Rossetta (DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal truncation of AMPKalpha at residue 312 yields a protein that is active upon phosphorylation of Thr172 in the absence of beta and gamma subunits, which is refered to as the AMPK catalytic domain and commonly used to substitute for AMPK heterotrimeric complex in in vitro kinase assays | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13131 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | activation of the enzyme by phosphorylation of Thr172 of the alpha subunit. Thr172 is the only site phosphorylated by its upstream kinase, liver kinase B1, and the phosphorylation dramatically increases the kinase activity of the catalytic domain/alpha-subunit. Phosphorylation of AMPK alpha1 on Thr172 by LKB1/Ste20-related adaptor (STRAD)/mouse protein 25(MO25) complex. Top-down mass spectrometric analysis, overview. Ser18 and Ser22 in the His-tag region as well as Thr196 (equivalent to Thr172 in the unmodified enzyme) in the sequence of the AMPK catalytic domain are the three phosphorylation sites | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-GGHMRSAMSGLHLVKRR-NH2 | i.e. SAMS peptide, a peptide derived from residues 73-85 of rat acetyl-CoA carboxylase in which Ser77 is mutated to Ala and the AMPK phosphorylation site is Ser79 | Homo sapiens | ADP + phosphorylated biotin-GGHMRSAMpSGLHLVKRR-NH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37953, recombinant phosphorylated AMPK alpha1 subunit, mass spectrometry | Homo sapiens |
heterotrimer | enzyme AMPK is a heterotrimeric protein complex composed of a catalytic subunit (alpha) and two regulatory subunits (beta and gamma) | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AMP-activated protein kinase | - |
Homo sapiens |
AMPK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase that is essential in regulating energy metabolism in all eukaryotic cells | Homo sapiens |