Literature summary for 2.7.11.31 extracted from

Gusarova, G.A.; Dada, L.A.; Kelly, A.M.; Brodie, C.; Witters, L.A.; Chandel, N.S.; Sznajder, J.I.
Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta (2009), Mol. Cell. Biol., 29, 3455-3464.

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Activating Compound

Activating Compound	Comment	Organism	Structure
O2	hypoxia leads to time-dependent AMPK activation in ATII cells. Maximal activation of AMPK after 10 min of 1.5% O2 exposure, whereas 3% O2 activates AMPK in a similar but slower manner. AMPK levels return to the baseline after 30 min of hypoxia exposure. Hypoxia-generated mitochondrial reactive oxygen species leads to the activation of the AMPK alpha1 isoform at Thr172. Hypoxia fails to activate AMPK in mitochondrion-deficient rho0-A549 cells	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
ATII cells infected with a hemagglutinin-tagged adenovirus carrying the dominant-negative mutant K45R of the AMPK alpha1 subunit or with an adenovirus expressing a constitutively active AMPK alpha variant or with an adenovirus expressing a constitutively active AMPK alpha variant in which Thr172 is replaced with aspartate in the truncated AMPK alpha subunit, comprising residues 1 to 312. A549 cells transfected with AMPK alpha1	Homo sapiens

Cloned (Comment)

Organism

ATII cells infected with a hemagglutinin-tagged adenovirus carrying the dominant-negative mutant K45R of the AMPK alpha1 subunit or with an adenovirus expressing a constitutively active AMPK alpha variant or with an adenovirus expressing a constitutively active AMPK alpha variant in which Thr172 is replaced with aspartate in the truncated AMPK alpha subunit, comprising residues 1 to 312. A549 cells transfected with AMPK alpha1

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K45R	dominant-negative, kinase-dead mutant of the AMPK alpha1 subunit	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo[1,5-a]-pyrimidine	compound C, hypoxia-induced PKCzeta translocation to the plasma membrane and phosphorylation at Thr410 is prevented by pharmacological inhibition of AMPK	Homo sapiens
additional information	overexpression of reactive oxygen species scavenger catalase prevents hypoxia-induced AMPK activation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
alveolar epithelium	-	Homo sapiens	-
additional information	ATII cell	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetyl-CoA carboxylase	AMPK alpha phosphorylates at Ser79	Homo sapiens	ADP + [acetyl-CoA carboxylase]phosphate	-	?
ATP + PKZeta	AMPK alpha phosphorylates PKZeta on residue Thr410 within the PKCzeta activation loop	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
AMP-activated protein kinase	-	Homo sapiens
AMPK	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	activation of AMPK alpha is necessary for hypoxia-induced AMPK-PKCzeta binding in alveolar epithelial cells. Overexpression of a dominant-negative AMPK alpha subunit construct prevents hypoxia-induced endocytosis of Na,K-ATPase, hypoxia-induced PKCzeta translocation to the plasma membrane and phosphorylation at Thr410	Homo sapiens