Literature summary for 2.7.11.31 extracted from

Iseli, T.J.; Oakhill, J.S.; Bailey, M.F.; Wee, S.; Walter, M.; van Denderen, B.J.; Castelli, L.A.; Katsis, F.; Witters, L.A.; Stapleton, D.; Macaulay, S.L.; Michell, B.J.; Kemp, B.E.
AMP-activated protein kinase subunit interactions: beta1:gamma1 association requires beta1 Thr-263 and Tyr-267 (2008), J. Biol. Chem., 283, 4799-4807.

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Activating Compound

Activating Compound	Comment	Organism	Structure
AMP	-	Rattus norvegicus
AMP	4.4fold activation at up to 0.08 mM	Homo sapiens
additional information	phosphorylation of AMPK activates the enzyme	Homo sapiens
additional information	phosphorylation of AMPK activates the enzyme	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GST-tagged wild-type AMPK beta1-alpha1 subunits fusion protein in COS-7 cells, and expression of beta1 residue 367 mutants in fusion to gamma1 subunit in COS-7 cells	Rattus norvegicus
expression of His6-tagged beta1(186-270)gamma1 complex and of GST-tagged isozyme AMPK alpha1beta1gamma1 in Spodoptera frugiperda Sf21 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Y267A	the mutation almost completely abrogates the beta1gamma1 interaction in comparison to wild type enzyme	Rattus norvegicus
Y267F	the mutation affects the beta1gamma1 interaction in comparison to wild type enzyme	Rattus norvegicus
Y267H	the mutation affects the beta1gamma1 interaction in comparison to wild type enzyme	Rattus norvegicus
Y267S	the mutation affects the beta1gamma1 interaction in comparison to wild type enzyme	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens
Mg2+	-	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation at alpha1-Thr172 by CaMKKbeta, and at alpha1-Ser485, beta1-Ser108, and beta1-Ser182 in Sf21 cells, determination of phosphorylation sites by mass spectrometry, phosphorylation also by LKB1, overview	Homo sapiens
phosphoprotein	phosphorylation by CaMKKbeta and LKB1, determination of phosphorylation sites by mass spectrometry, overview	Rattus norvegicus

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged isozyme AMPK alpha1beta1gamma1 from Spodoptera frugiperda Sf21 cells by glutathione affinity and anion exchange chromatography, and gel filtration	Homo sapiens
recombinant GST-tagged isozyme AMPK beta1-alpha1 subunits fusion protein from COS-7 cells by glutathione affinity exchange chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.6	-	purified recombinant enzyme	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [peptide HMRSAMSGLHLVKRR]	i.e. SAMS peptide	Homo sapiens	ADP + [peptide HMRSAMSGLHLVKRR] phosphate	-	?
ATP + [peptide HMRSAMSGLHLVKRR]	i.e. SAMS peptide	Rattus norvegicus	ADP + [peptide HMRSAMSGLHLVKRR] phosphate	-	?

Subunits

Subunits	Comment	Organism
More	AMPK consists of a catalytic alpha subunit and two non-catalytic subunits,beta and gamma, each with multiple isoforms that form active 1:1:1 heterotrimers	Homo sapiens
More	AMPK consists of a catalytic alpha subunit and two non-catalytic subunits,beta and gamma, each with multiple isoforms that form active 1:1:1 heterotrimers, beta1-Thr263 and beta1-Tyr267 are required for beta1gamma1 subunit association	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
AMP-activated protein kinase	-	Homo sapiens
AMP-activated protein kinase	-	Rattus norvegicus
AMPK	-	Homo sapiens
AMPK	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens
30	-	assay at	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens
7.5	-	assay at	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens
ATP	-	Rattus norvegicus

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Release 2023.1 (January 2023)