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Literature summary for 2.7.11.31 extracted from

  • Short, J.D.; Houston, K.D.; Dere, R.; Cai, S.L.; Kim, J.; Johnson, C.L.; Broaddus, R.R.; Shen, J.; Miyamoto, S.; Tamanoi, F.; Kwiatkowski, D.; Mills, G.B.; Walker, C.L.
    AMP-activated protein kinase signaling results in cytoplasmic sequestration of p27 (2008), Cancer Res., 68, 6496-6506.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.31

Activating Compound

Activating Compound Comment Organism Structure
AMP involved in AMPK phosphorylation Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of HA-tagged AMPK alpha1 subunit in NIH3T3 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information cells deficient in Tsc2, a tuberin encoded by gene tsc2, show cytoplasmic mislocalization of p27, which is reversible by inhibitors of the LKB1/AMPK pathway Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
compound C i.e. dorsomorphin or 6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo[1,5-a]-pyrimidine, a specific inhibitor of AMPK Mus musculus
additional information no inhibition by LY294002 and PD98059 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Mus musculus 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + histone H1B Mus musculus
-
 ADP + phospho-histone H1B
-
 ?
ATP + p27 Mus musculus loss of tuberin is associated with increased AMPK activity and altered p27 function leading to increased Cdk2 activity and resistance of the cells against apoptosis. Mislocation of p27 occurs in tuberin-deficient cells, possessing no functional gene tsc2, and can induced directly by activating AMPK physiologically via glucose deprivation or genetically via a constitutively active AMPK, overview ADP + phospho-p27
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein AMPK alpha1 subunit is phosphorylated at Thr172 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H1B
-
 Mus musculus ADP + phospho-histone H1B
-
 ?
ATP + p27 loss of tuberin is associated with increased AMPK activity and altered p27 function leading to increased Cdk2 activity and resistance of the cells against apoptosis. Mislocation of p27 occurs in tuberin-deficient cells, possessing no functional gene tsc2, and can induced directly by activating AMPK physiologically via glucose deprivation or genetically via a constitutively active AMPK, overview Mus musculus ADP + phospho-p27
-
 ?
ATP + p27 AMPK phosphorylates p27 function at least at three sites, Thr172, Thr170, and Ser83, Thr170 is localized near the nuclear localization signal sequence and its phosphorylation is responsible for p27 translocation to the cytoplasm Mus musculus ADP + phospho-p27
-
 ?

Synonyms

Synonyms Comment Organism
AMP-activated protein kinase
-
 Mus musculus
AMPK
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus