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Literature summary for 2.7.11.31 extracted from

  • Woods, A.; Vertommen, D.; Neumann, D.; Tuerk, R.; Bayliss, J.; Schlattner, U.; Wallimann, T.; Carling, D.; Rider, M.H.
    Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis (2003), J. Biol. Chem., 278, 28434-28442.
    View publication on PubMed
Search for more literature for 2.7.11.31

Activating Compound

Activating Compound Comment Organism Structure
5'-AMP
-
 Rattus norvegicus
5-amino-4-imidazolecarboxamide ribonucleoside
-
 Rattus norvegicus
adiponectin
-
 Rattus norvegicus
leptin
-
 Rattus norvegicus
metformin antidiabetic drug Rattus norvegicus
additional information AMPK can also be activated by hyperosmotic stress Rattus norvegicus
rosiglitazone antidiabetic drug Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
transfection of CCL13 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
S485A site-directed mutagenesis, non-phosphorylatable mutant Rattus norvegicus
S485D site-directed mutagenesis Rattus norvegicus
T172E site-directed mutagenesis Rattus norvegicus
T258A site-directed mutagenesis, non-phosphorylatable mutant Rattus norvegicus
T258D site-directed mutagenesis Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 63000 + 1 * 40000 + ?, alpha and beta subunit, SDS-PAGE Rattus norvegicus
63000
-
 1 * 63000 + 1 * 40000 + ?, alpha and beta subunit, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus activated AMPK acts to down-regulate ATP-consuming pathways such as fatty acid synthesis by phosphorylating and inactivating acetyl-CoA carboxylase and protein synthesis by promoting the phosphorylation of eukaryotic elongation factor-2, in heart AMPK activation stimulates glycolysis by increasing glucose uptake ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes, expressed in bacteria Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone 2A
-
 Rattus norvegicus ?
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] activated AMPK acts to down-regulate ATP-consuming pathways such as fatty acid synthesis by phosphorylating and inactivating acetyl-CoA carboxylase and protein synthesis by promoting the phosphorylation of eukaryotic elongation factor-2, in heart AMPK activation stimulates glycolysis by increasing glucose uptake Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 63000 + 1 * 40000 + ?, alpha and beta subunit, SDS-PAGE Rattus norvegicus
More heterotrimeric serine/threonine kinase consists of a catalytic alpha-subunit and 2 regulatory subunits beta and gamma, each subunit exists as multiple isoforms, alpha1, alpha2, beta1, beta2, gamma1, gamma2 and gamma3, giving 12 different possible combinantions of holoenzyme with different tissue distribution and subcellular localization Rattus norvegicus