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Literature summary for 2.7.11.31 extracted from

  • Beg, Z.H.; Stonik, J.A.; Brewer, H.B.
    Phosphorylation and modulation of the enzymic activity of native and protease-cleaved purified hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase by a calcium/calmodulin-dependent protein kinase (1987), J. Biol. Chem., 262, 13228-13240.
    View publication on PubMed
Search for more literature for 2.7.11.31

General Stability

General Stability Organism
highly labile enzyme Rattus norvegicus
protease inhibitors stabilizes enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EGTA
-
 Rattus norvegicus
Inhibitor W-7 specific Ca2+/calmodulin-dependent kinase inhibitor Rattus norvegicus
Trifluperazine specific Ca2+/calmodulin-dependent kinase inhibitor Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00085
-
 hydroxymethylglutaryl-CoA reductase pH 6.5, 30°C, low-MW kinase Rattus norvegicus
0.0029
-
 histone H1 pH 6.5, 30°C, low-MW kinase Rattus norvegicus
0.028
-
 ATP pH 6.5, 30°C, low-MW kinase Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000 125000 low-MW kinase, gel filtration Rattus norvegicus
560000 600000 high-MW kinase, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus inactivates EC 1.1.1.34 by phosphorylation ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
low-MW kinase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.292
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein relative kinase activity for low-MW kinase 8%, high MW-kinase 48% Rattus norvegicus ADP + casein phosphate
-
 ?
ATP + casein relative kinase activity for low-MW kinase 8%, high MW-kinase 48% Rattus norvegicus Sprague-Dawley ADP + casein phosphate
-
 ?
ATP + dephospho-alpha,beta-tubulin relative kinase activity high MW-kinase 15% Rattus norvegicus ADP + [alpha,beta-tubulin] phosphate
-
 ?
ATP + glycogen synthase relative kinase activity for low-MW kinase 7%, high MW-kinase 87% Rattus norvegicus ADP + [glycogen synthase] phosphate
-
 ?
ATP + heavy meromyosin relative kinase activity for low-MW kinase 2%, high MW-kinase 100% Rattus norvegicus ADP + [heavy meromyosin] phosphate
-
 ?
ATP + histone H1
-
 Rattus norvegicus ADP + phosphohistone H1
-
 ?
ATP + histone H1 (IIIS) histones are better substrates for high-MW kinase than hydroxymethylglutaryl-CoA reductase, relative kinase activity for low-MW kinase 275%, high MW-kinase 103% Rattus norvegicus ADP + [histone H1 (IIIS)] phosphate
-
 ?
ATP + histone II-S relative kinase activity for low-MW kinase 38%, high MW-kinase 159% Rattus norvegicus ADP + [histone II-S] phosphate
-
 ?
ATP + histone VIIIS relative kinase activity for low-MW kinase 65%, high MW-kinase 141% Rattus norvegicus ADP + [histone VIIIS] phosphate
-
 ?
ATP + MAP-2 relative kinase activity for low-MW kinase 14%, high MW-kinase 566% Rattus norvegicus ADP + MAP-2 phosphate
-
 ?
ATP + myelin basic protein moderate substrate for low-MW kinase, better than hydroxymethylglutaryl-CoA reductase for high-MW kinase, relative kinase activity for low-MW kinase 36%, high MW-kinase 238% Rattus norvegicus ADP + [myelin basic protein] phosphate
-
 ?
ATP + myosin mixed light chains relative kinase activity for low-MW kinase 4%, high MW-kinase 27% Rattus norvegicus ADP + [myosin mixed light chains] phosphate
-
 ?
ATP + phosphorylase B relative kinase activity high MW-kinase 12% Rattus norvegicus ADP + [phosphorylase B] phosphate
-
 ?
ATP + phosvitin relative kinase activity for low-MW kinase 2%, high MW-kinase 2% Rattus norvegicus ADP + phosvitin phosphate
-
 ?
ATP + phosvitin relative kinase activity for low-MW kinase 2%, high MW-kinase 2% Rattus norvegicus Sprague-Dawley ADP + phosvitin phosphate
-
 ?
ATP + protamine relative kinase activity for low-MW kinase 24%, high MW-kinase 38% Rattus norvegicus ADP + protamine phosphate
-
 ?
ATP + protamine relative kinase activity for low-MW kinase 24%, high MW-kinase 38% Rattus norvegicus Sprague-Dawley ADP + protamine phosphate
-
 ?
ATP + synapsin 1 as good substrate as hydroxymethylglutaryl-CoA reductase, relative kinase activity for low-MW kinase 151%, high MW-kinase 103% Rattus norvegicus ADP + [synapsin 1] phosphate
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
 Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] inactivates EC 1.1.1.34 by phosphorylation Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
additional information autophosphorylation in absence of substrate Rattus norvegicus ?
-
 ?
additional information incorporates 0.5 mol phosphate/mol MW 53000 enzyme substrate fragment, 2 mol phosphate/mol native enzyme substrate Rattus norvegicus ?
-
 ?
additional information autophosphorylation in absence of substrate Rattus norvegicus Sprague-Dawley ?
-
 ?
additional information incorporates 0.5 mol phosphate/mol MW 53000 enzyme substrate fragment, 2 mol phosphate/mol native enzyme substrate Rattus norvegicus Sprague-Dawley ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 low-MW kinase Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 about half-maximal activity at pH 5.5 and 7.0, low-MW kinase Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
Calmodulin requirement, Ca2+/calmodulin dependent kinase, no phosphorylation of substrate observed in absence Rattus norvegicus