BRENDA - Enzyme Database show
show all sequences of 2.7.11.30

Smad2 phosphorylation by type I receptor. Contribution of arginine 462 and cysteine 463 In the C terminus of Smad2 for specificity

Yakymovych, I.; Heldin, C.H.; Souchelnytskyi, S.; J. Biol. Chem. 279, 35781-35787 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
TGF-beta
a potent regulator of cell proliferation, differentiation, motility, and apoptosis, binds to and activates serine/threonine receptors that phosphorylate Smad2 and Smad3
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Mus musculus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Mus musculus
Mn2+
-
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + Smad2
Mus musculus
-
ADP + phosphorylated Smad2
-
-
?
ATP + Smad3
Mus musculus
-
ADP + phosphorylated Smad3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fibroblast
-
Mus musculus
-
MEF cell
enbryonic fibroblast cell line
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + Smad2
-
665554
Mus musculus
ADP + phosphorylated Smad2
-
-
-
?
ATP + Smad2
TGF-beta-induced phosphorylation of Arg462 and Cys463 by TbetaR-I, no activity with Smad2 mutant R462I/C463A by TbetaR-I, Smad2 is no substrate of TbetaR-II and BMP type II receptor
665554
Mus musculus
ADP + phosphorylated Smad2
-
-
-
?
ATP + Smad3
-
665554
Mus musculus
ADP + phosphorylated Smad3
-
-
-
?
ATP + Smad3
TGF-beta-induced C-terminal phosphorylation
665554
Mus musculus
ADP + phosphorylated Smad3
-
-
-
?
additional information
substrate specificity of transforming growth factor-beta receptor I
665554
Mus musculus
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Mus musculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
TGF-beta
a potent regulator of cell proliferation, differentiation, motility, and apoptosis, binds to and activates serine/threonine receptors that phosphorylate Smad2 and Smad3
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Mus musculus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Mus musculus
Mn2+
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + Smad2
Mus musculus
-
ADP + phosphorylated Smad2
-
-
?
ATP + Smad3
Mus musculus
-
ADP + phosphorylated Smad3
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fibroblast
-
Mus musculus
-
MEF cell
enbryonic fibroblast cell line
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + Smad2
-
665554
Mus musculus
ADP + phosphorylated Smad2
-
-
-
?
ATP + Smad2
TGF-beta-induced phosphorylation of Arg462 and Cys463 by TbetaR-I, no activity with Smad2 mutant R462I/C463A by TbetaR-I, Smad2 is no substrate of TbetaR-II and BMP type II receptor
665554
Mus musculus
ADP + phosphorylated Smad2
-
-
-
?
ATP + Smad3
-
665554
Mus musculus
ADP + phosphorylated Smad3
-
-
-
?
ATP + Smad3
TGF-beta-induced C-terminal phosphorylation
665554
Mus musculus
ADP + phosphorylated Smad3
-
-
-
?
additional information
substrate specificity of transforming growth factor-beta receptor I
665554
Mus musculus
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mus musculus
Other publictions for EC 2.7.11.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
1
1
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1
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Reduced SMAD7 leads to overact ...
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71
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2011
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1
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-
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1
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2
2
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702898
Haneen
Mutation analysis of 'Endoglin ...
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BMC Med. Genet.
10
53
2009
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1
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-
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1
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1
1
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703244
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Rattus norvegicus
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119
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2009
-
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-
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2
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2
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2
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1
1
1
1
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704485
Upton
Bone morphogenetic protein (BM ...
Homo sapiens
J. Biol. Chem.
284
15794-15804
2009
-
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1
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1
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1
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1
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1
1
1
1
-
-
705441
Mahmoud
Endoglin and activin receptor- ...
Mus musculus
Lab. Invest.
89
15-25
2009
-
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-
-
-
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-
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-
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4
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6
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6
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1
2
2
1
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-
690773
Watanabe
Insulin gene is a target in ac ...
Homo sapiens
Biochem. Biophys. Res. Commun.
377
867-872
2008
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1
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1
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5
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1
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2
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691269
Kim
Synthesis and biological evalu ...
Rattus norvegicus
Bioorg. Med. Chem. Lett.
18
2122-2127
2008
-
-
-
-
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52
-
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1
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52
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691460
Zhou
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112
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-
-
-
-
1
-
1
-
-
1
-
2
-
2
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4
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2
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1
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1
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4
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2
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691532
Xu
TGF-beta receptor kinase inhib ...
Homo sapiens
Br. J. Haematol.
142
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2008
-
-
-
-
-
-
1
-
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1
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2
-
2
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6
-
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3
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1
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1
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1
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6
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3
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691833
Fujiwara
Implications of mutations of a ...
Homo sapiens
Circ. J.
72
127-133
2008
-
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1
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5
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2
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1
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5
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2
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1
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692159
Bueno
Semi-mechanistic modelling of ...
Homo sapiens
Eur. J. Cancer
44
142-150
2008
-
-
-
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1
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1
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1
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4
-
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6
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1
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1
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6
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1
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693034
Zhao
Smad4-dependent TGF-beta signa ...
Homo sapiens
J. Biol. Chem.
283
11293-11301
2008
-
-
-
-
-
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1
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2
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1
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7
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3
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1
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7
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3
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693381
Young
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Ovis aries
J. Endocrinol.
196
251-261
2008
-
-
-
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1
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694090
Xu
Cyclin G2 is degraded through ...
Homo sapiens
Mol. Biol. Cell
19
4968-4979
2008
-
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706906
Li
Down-regulation of transformin ...
Homo sapiens
World J. Gastroenterol.
14
2894-2899
2008
-
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2
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2
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664721
Zhang
Activin receptor-like kinase 7 ...
Rattus norvegicus
Diabetologia
49
506-518
2006
3
-
1
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1
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3
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2
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5
-
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4
1
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3
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1
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1
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664088
Peng
Kinetic characterization of no ...
Homo sapiens
Biochemistry
44
2293-2304
2005
-
-
1
-
1
-
4
4
-
1
-
-
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2
-
1
1
-
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9
1
1
-
-
-
1
-
-
1
3
-
4
-
-
1
1
-
1
-
4
4
3
4
-
1
-
-
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1
1
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-
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-
9
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
664715
Desgrosellier
Activin receptor-like kinase 2 ...
Gallus gallus
Dev. Biol.
280
201-210
2005
1
-
1
-
1
-
-
-
-
-
-
1
-
1
-
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-
-
-
3
-
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2
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1
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1
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1
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1
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3
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2
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665640
Ungefroren
Transforming growth factor-bet ...
Homo sapiens
J. Biol. Chem.
280
2644-2652
2005
1
-
-
-
-
-
-
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1
-
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2
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1
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4
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3
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1
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1
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1
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4
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3
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-
665699
Lux
Human retroviral gag- and gag- ...
Homo sapiens
J. Biol. Chem.
280
8482-8493
2005
1
-
1
-
1
-
1
-
1
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-
-
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2
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1
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1
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1
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1
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1
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1
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1
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666443
Halder
A specific inhibitor of TGF-be ...
Homo sapiens
Neoplasia
7
509-521
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1
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1
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2
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1
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1
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1
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1
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1
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663984
Ge
Selective inhibitors of type I ...
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3
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3
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1
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1
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1
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2
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1
1
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1
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1
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2
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3
-
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3
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1
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1
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664036
Ottesen
Semisynthesis of phosphovarian ...
Homo sapiens
Biochemistry
43
5698-5706
2004
2
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1
2
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2
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2
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4
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1
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1
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1
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2
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1
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1
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2
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4
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1
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1
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664318
Sawyer
Synthesis and activity of new ...
Homo sapiens, Mus musculus
Bioorg. Med. Chem. Lett.
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3581-3584
2004
2
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1
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6
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2
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2
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2
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1
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2
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2
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2
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2
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2
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6
2
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1
2
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6
6
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2
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2
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1
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2
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2
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2
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2
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665532
Kim
Activin receptor-like kinase-7 ...
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3
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2
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5
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1
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1
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1
1
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3
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1
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2
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5
-
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665554
Yakymovych
Smad2 phosphorylation by type ...
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J. Biol. Chem.
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1
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2
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5
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1
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1
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1
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1
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1
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1
2
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2
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2
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5
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1
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1
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666283
Forrester
Tegumental expression of a nov ...
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149-156
2004
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1
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4
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1
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1
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4
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-
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666359
Mazerbourg
Growth differentiation factor- ...
Rattus norvegicus
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653-665
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2
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1
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1
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2
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2
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3
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4
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1
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2
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1
1
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1
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1
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3
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3
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4
-
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664282
Roberts
Identification of novel isofor ...
Homo sapiens
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2003
2
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1
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1
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2
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8
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2
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1
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1
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1
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8
-
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665481
Liu
Smads 2 and 3 are differential ...
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1
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1
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5
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1
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5
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665487
Harrison
Identification of a functional ...
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1
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1
1
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1
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2
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1
1
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27
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1
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1
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1
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1
1
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666312
Goumans
Activin receptor-like kinase ( ...
Mus musculus
Mol. Cell
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817-828
2003
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1
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5
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1
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2
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1
1
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1
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1
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1
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5
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1
1
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5
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666512
Fink
TGF-beta-induced nuclear local ...
Homo sapiens
Oncogene
22
1317-1323
2003
1
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1
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3
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1
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5
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5
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1
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1
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1
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1
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5
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