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Literature summary for 2.7.11.28 extracted from
- Isolation and characterization of tropomyosin kinase from chicken embryo (1987), Biochim. Biophys. Acta, 925, 17-26.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | requirement | Gallus gallus |  |
General Stability
| General Stability | Organism |
|---|---|
| highly purified enzyme is very unstable upon further purification | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by heparin | Gallus gallus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
alpha-tropomyosin | 37°C, pH 7.5, skeletal alpha-tropomyosin | Gallus gallus | |
| 0.2 | - |
ATP | 37°C, pH 7.5 | Gallus gallus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Gallus gallus | |
| additional information | no activation by Ca2+ | Gallus gallus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 252000 | - |
gel filtration | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
| additional information | not in adult chicken or adult rabbit skeletal muscle | Gallus gallus | - |
| additional information | not in adult chicken or adult rabbit skeletal muscle | Oryctolagus cuniculus | - |
| muscle | thigh and leg | Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0594 | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 10% sucrose at least 2 weeks | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | - |
Gallus gallus | ADP + phosphocasein | - |
? | |
| ATP + histone II-A | - |
Gallus gallus | ADP + phosphohistone II-A | - |
? | |
| ATP + phosphorylase b | - |
Gallus gallus | ADP + phosphophosphorylase b | - |
? | |
| ATP + phosvitin | - |
Gallus gallus | ADP + phosphophosvitin | - |
? | |
| ATP + tropomyosin | beta-tropomyosin from turkey gizzard | Gallus gallus | ADP + O-phosphotropomyosin | - |
? | |
| ATP + tropomyosin | alpha-tropomyosin subunit preferred over beta-tropomyosin subunit | Gallus gallus | ADP + O-phosphotropomyosin | - |
? | |
| ATP + troponin complex | from rabbit skeletal muscle | Gallus gallus | ADP + phosphotroponin complex | - |
? | |
| additional information | no substrates are smooth muscle myosin light chain from turkey gizzard, tropomyosin from platelet and erythrocyte from chicken gizzard and smooth muscle | Gallus gallus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
about | Gallus gallus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 40 | about 10% of maximal activity at 22°C and about 60% of maximal activity at 40°C | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 9.5 | about half-maximal activity at pH 6.2 and 9.5 | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by cAMP or calmodulin | Gallus gallus |
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