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Literature summary for 2.7.11.26 extracted from

  • Kitano-Takahashi, M.; Morita, H.; Kondo, S.; Tomizawa, K.; Kato, R.; Tanio, M.; Shirota, Y.; Takahashi, H.; Sugio, S.; Kohno, T.
    Expression, purification and crystallization of a human tau-tubulin kinase 2 that phosphorylates tau protein (2007), Acta Crystallogr. Sect. F, 63, 602-604.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
TTBK2 kinase domain (residues 1-331) of TTBK2 subcloned into pBiEx-3 vector, expressed in insect cells with a baculovirus overexpression system Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
by hanging-drop vapour-diffusion method, crystals belong to space group P212121, with unit-cell parameters a = 55.6, b = 113.7, c = 117.3 A, alpha = beta = gamma = 90.0°, to 2.9 A resolution Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Homo sapiens
38620
-
calculated Homo sapiens
39000
-
1 * 39000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation, sonication, gel filtration, metal affinity chromatography, to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein tau TTBK2 (1-331) phosphorylates residues Ser208 and Ser210 Homo sapiens ADP + protein tau phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 39000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
tau-tubulin kinase 2
-
Homo sapiens
TTBK2
-
Homo sapiens