Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.25 extracted from

  • Yue, J.; Xiong, W.; Ferrell, J.E.
    B-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase activation in Xenopus egg extracts (2006), Oncogene, 1, 1-9.
    View publication on PubMed
Search for more literature for 2.7.11.25

Activating Compound

Activating Compound Comment Organism Structure
Ras activates C-Raf and B-Raf Xenopus laevis

Cloned(Commentary)

Cloned (Comment) Organism
B-RAf, DNA and amino acid sequence determination and analysis, expression of His6-tagged B-Raf in bacteria, expression of His6-tagged and FLAG-tagged C-Raf in insect cells Xenopus laevis

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus activation of MEK1 by C-Raf Xenopus laevis 5794
-
kinetochore activation of p42 MAPK Xenopus laevis 776
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + MEK1 Xenopus laevis Ras-induced activation of the MAPK signaling cascade ADP + phosphorylated MEK1
-
 ?
ATP + p42 MAPK Xenopus laevis Ras-induced activation of the MAPK signaling cascade ADP + phosphorylated p42 MAPK
-
 ?
additional information Xenopus laevis B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of Mos and B-Raf from egg extracts by heparin affinity chromatography and/or ion exchange chromatography, and GST-MEK1 affinity chromatography, recombinant His6-tagged B-Raf by nickel affinity chromatography Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
egg no changes in B-Raf activity after fertilization Xenopus laevis
-
embryo no changes in B-Raf activity in the first embryonic cell cycles Xenopus laevis
-
oocyte no changes in B-Raf activity during progesterone-induced maturation Xenopus laevis
-
zygote
-
 Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H1
-
 Xenopus laevis ADP + phosphorylated histone H1
-
 ?
ATP + MEK1 Ras-induced activation of the MAPK signaling cascade Xenopus laevis ADP + phosphorylated MEK1
-
 ?
ATP + MEK1 the substrate of B-Raf and C-Raf is a MAPKK, no substrate of Mos, recombinant GST-MEK1 substrate Xenopus laevis ADP + phosphorylated MEK1
-
 ?
ATP + p42 MAPK Ras-induced activation of the MAPK signaling cascade Xenopus laevis ADP + phosphorylated p42 MAPK
-
 ?
ATP + p42 MAPK the substrate of B-Raf and C-Raf, no substrate of Mos, recombinant His6-p42 MAPK substrate Xenopus laevis ADP + phosphorylated p42 MAPK
-
 ?
additional information B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation Xenopus laevis ?
-
 ?

Synonyms

Synonyms Comment Organism
B-Raf
-
 Xenopus laevis
C-Raf
-
 Xenopus laevis
MAP kinase kinase kinase
-
 Xenopus laevis
MAPKKK
-
 Xenopus laevis
Mos
-
 Xenopus laevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Xenopus laevis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Xenopus laevis