Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | a functional DVD docking site of MAPKK substrates is absolutely required for activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
co-expression of FLAG-tagged MTK1 and wild-type and mutant HA-tagged MKK6 in COS-7 cells, direct and immunoprecipitation study | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E1372Q | site-directed mutagenesis, the MTK1 mutant shows altered substrate specificity comapred to the wild-type enzyme | Homo sapiens |
K1371D | site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants | Homo sapiens |
K1371E | site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants | Homo sapiens |
K1371G | site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants | Homo sapiens |
K1371R | site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants | Homo sapiens |
Q1254E | site-directed mutagenesis, the MEKK1 mutant shows altered substrate specificity comapred to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | MAPKK with mutated DVD docking sites are inhibitory for the MAPKKK, as well as synthetic DVD sequence oligopeptides of e.g. MKK6, in vitro and in vivo, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK6 | Homo sapiens | substrate of stress-activated MAPKKKs ASK1 and MTK1 | ADP + phosphorylated MKK6 | - |
? | |
additional information | Homo sapiens | the MAPKKKs are part of the MAPK signaling cascade, several structurally diverse MAPKKK families exist, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | structure-function relationship of MAPKKKs and MAPKKs, K1371 is the catalytic residue of MTK1, MTK1, TAK1, and MEKK1 also possess lysine as catalytic residues | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK3 | - |
Homo sapiens | ADP + phosphorylated MKK3 | - |
? | |
ATP + MKK4 | substrate of MEKK1 mutant Q1254E and of MTK1 mutant E1372Q | Homo sapiens | ADP + phosphorylated MKK4 | - |
? | |
ATP + MKK6 | substrate of stress-activated MAPKKKs ASK1 and MTK1 | Homo sapiens | ADP + phosphorylated MKK6 | - |
? | |
ATP + MKK6 | phosphorylation in the activation loop, substrate of stress-activated MAPKKKs ASK1 and MTK1, no activity with MKK6 mutants E318P, F327D, I331D, and V328G, but reduced activity with MKK6 mutants V324G and V328A | Homo sapiens | ADP + phosphorylated MKK6 | - |
? | |
additional information | the MAPKKKs are part of the MAPK signaling cascade, several structurally diverse MAPKKK families exist, overview | Homo sapiens | ? | - |
? | |
additional information | substrate specificities of MAPKKKs with wild-type and mutant MAPKKs, overview, no activity with MKK7 by MEKK1 mutant Q1254E, wild-type MTK1, and MTK1 mutant E1372Q, the enzyme docks at the DVD docking site of MAPKK, a stretch of about 20 amino acids immediately on the C-terminal side of the catalytic domain, MAPKK with mutated DVD docking sites are inhibitory for the MAPKKK, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASK1 | - |
Homo sapiens |
MAP kinase kinase kinase | - |
Homo sapiens |
MAPKKK | - |
Homo sapiens |
MEKK1 | - |
Homo sapiens |
MEKK2 | - |
Homo sapiens |
MEKK3 | - |
Homo sapiens |
MEKK4 | - |
Homo sapiens |
MLK | - |
Homo sapiens |
Mos | - |
Homo sapiens |
MTK1 | - |
Homo sapiens |
Raf | - |
Homo sapiens |
TAK1 | - |
Homo sapiens |
TAO | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |