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Literature summary for 2.7.11.25 extracted from

  • Kwan, J.J.; Warner, N.; Maini, J.; Chan Tung, K.W.; Zakaria, H.; Pawson, T.; Donaldson, L.W.
    Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction (2006), J. Mol. Biol., 356, 142-154.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Ste50 Ste50 binding of the MAPKKK Ste11 through a head-to-tail sterile interaction via both alpha motif SAM domains is required for Ste11 activity and cell viability, NMR binding study, very tight and stable binding between the two mutants Ste50 L69R and Ste11 L72R, molecular modeling, overview Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His6- and GST-tagged wild-type and mutants enzymes in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A62D site-directed mutagenesis, no binding of Ste50 Saccharomyces cerevisiae
I59R site-directed mutagenesis, no binding of Ste50 Saccharomyces cerevisiae
L72R site-directed mutagenesis, highly increased tight binding of Ste50 wild-type compared to the Ste11 wild-type enzyme Saccharomyces cerevisiae
additional information inhibition of heterodimeric association of Ste11 and Ste50 in yeast strains leads to defects in mating and activation of high-osmolarity growth pathways Saccharomyces cerevisiae
Y54R site-directed mutagenesis, no binding of Ste50 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of binding between wild-type and mutant Ste11 and wild-type and mutant Ste50, overview Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae Ste11 is involved in MAPK pathway signal transduction governing mating, osmoregulation, and nitrogen starvation by direct interaction with the sterile alpha motif domains, SAM, of Ste50 and Ste11 ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6- and GST-tagged wild-type and mutants enzymes from Escherichia coli by affinity chromatography and gel filtration, the tags are cleaved off Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Ste11 is involved in MAPK pathway signal transduction governing mating, osmoregulation, and nitrogen starvation by direct interaction with the sterile alpha motif domains, SAM, of Ste50 and Ste11 Saccharomyces cerevisiae ?
-
?
additional information Ste50 binds the MAPKKK Ste11 through a head-to-tail sterile interaction via both alpha motif SAM domains, NMR binding study, very tight and stable binding between the two mutants Ste50 L69R and Ste11 L72R, overview Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
More structural analysis of the sterile alpha motif, SAM, domain of Ste11 MAPKKK and of Ste50 effector, several hydrophobic secondary structures and amino acids are required for effective binding, overview Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
MAPKKK
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Saccharomyces cerevisiae
Ste11
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae