Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ste50 | Ste50 binding of the MAPKKK Ste11 through a head-to-tail sterile interaction via both alpha motif SAM domains is required for Ste11 activity and cell viability, NMR binding study, very tight and stable binding between the two mutants Ste50 L69R and Ste11 L72R, molecular modeling, overview | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
overexpression of His6- and GST-tagged wild-type and mutants enzymes in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A62D | site-directed mutagenesis, no binding of Ste50 | Saccharomyces cerevisiae |
I59R | site-directed mutagenesis, no binding of Ste50 | Saccharomyces cerevisiae |
L72R | site-directed mutagenesis, highly increased tight binding of Ste50 wild-type compared to the Ste11 wild-type enzyme | Saccharomyces cerevisiae |
additional information | inhibition of heterodimeric association of Ste11 and Ste50 in yeast strains leads to defects in mating and activation of high-osmolarity growth pathways | Saccharomyces cerevisiae |
Y54R | site-directed mutagenesis, no binding of Ste50 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of binding between wild-type and mutant Ste11 and wild-type and mutant Ste50, overview | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Ste11 is involved in MAPK pathway signal transduction governing mating, osmoregulation, and nitrogen starvation by direct interaction with the sterile alpha motif domains, SAM, of Ste50 and Ste11 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6- and GST-tagged wild-type and mutants enzymes from Escherichia coli by affinity chromatography and gel filtration, the tags are cleaved off | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ste11 is involved in MAPK pathway signal transduction governing mating, osmoregulation, and nitrogen starvation by direct interaction with the sterile alpha motif domains, SAM, of Ste50 and Ste11 | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Ste50 binds the MAPKKK Ste11 through a head-to-tail sterile interaction via both alpha motif SAM domains, NMR binding study, very tight and stable binding between the two mutants Ste50 L69R and Ste11 L72R, overview | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural analysis of the sterile alpha motif, SAM, domain of Ste11 MAPKKK and of Ste50 effector, several hydrophobic secondary structures and amino acids are required for effective binding, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
MAPKKK | - |
Saccharomyces cerevisiae |
Ste11 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |