Literature summary for 2.7.11.25 extracted from

Machida, N.; Umikawa, M.; Takei, K.; Sakima, N.; Myagmar, B.E.; Taira, K.; Uezato, H.; Ogawa, Y.; Kariya, K.
Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase (2004), J. Biol. Chem., 279, 15711-15714.

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Activating Compound

Activating Compound	Comment	Organism	Structure
H2O2	oxidative stress activates OMTK1	Medicago sativa
additional information	MG115 induces OMTK1	Medicago sativa
NaCl	-	Medicago sativa

Cloned(Commentary)

Cloned (Comment)	Organism
OMTK1, DNA and amino acid sequence determination, expression as GST-fusion protein, transient co-expression of Myc-tagged OMTK1 and HA-tagged MMK3 in Arabidopsis thaliana protoplasts, in vitro trabscription and translation of wild-type and mutant OMTK1 by reticulocyte lysate	Medicago sativa

Protein Variants

Protein Variants	Comment	Organism
additional information	an OMTK1 mutant reduced in MMK3 complex formation shows decreased MMK3 and cell death activation	Medicago sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
protoplast	basal activity level of OMTK1	Medicago sativa	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + MKK3	Medicago sativa	a MAPKK, OMTK1 channels oxidative stress signaling through direct interaction with MAPK MMK3 increasing the cell death rate, OMTK1 and MMK3 form a complex in vivo	ADP + phosphorylated MKK3	-	?
additional information	Medicago sativa	the OMTK1 kinase domain is involved in activation of signaling pathways in case of oxidative stress to induce cell death playing a MAPK scaffolding role, OMTK1 shows low constitutive activity	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Medicago sativa	Q7XTK4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + MKK3	a MAPKK	Medicago sativa	ADP + phosphorylated MKK3	-	?
ATP + MKK3	a MAPKK, OMTK1 channels oxidative stress signaling through direct interaction with MAPK MMK3 increasing the cell death rate, OMTK1 and MMK3 form a complex in vivo	Medicago sativa	ADP + phosphorylated MKK3	-	?
additional information	the OMTK1 kinase domain is involved in activation of signaling pathways in case of oxidative stress to induce cell death playing a MAPK scaffolding role, OMTK1 shows low constitutive activity	Medicago sativa	?	-	?

Synonyms

Synonyms	Comment	Organism
MAPKKK	-	Medicago sativa
MEKK1	-	Medicago sativa
OMTK1	-	Medicago sativa
oxidative stress-activated MAP triple-kinase 1	-	Medicago sativa
putative mitogen-activated protein kinase 1	-	Medicago sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Medicago sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Medicago sativa

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Medicago sativa

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Release 2023.1 (January 2023)