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Literature summary for 2.7.11.24 extracted from
- ATLAS - a high-throughput affinity-based screening technology for soluble proteins: technology application using p38 MAP kinase (2008), Assay Drug Dev. Technol., 6, 55-68.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | development and evaluation of a high-throughput affinity-based screening technology, ATLAS, for soluble proteins, e.g. p38 MAP kinase, effects of p38 MAP kinase inhibitors, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-(4-chlorphenyl)-4-(4-fluorophenyl)-5-pyridin-4-yl-1,2-dihydropyrazol-3-one | - |
Homo sapiens |  |
| additional information | effects of inhibitors on ATLAS technology assay, overview | Homo sapiens | |
| PD169316 | - |
Homo sapiens | |
| SB202190 | - |
Homo sapiens | |
| SB203580 | - |
Homo sapiens | |
| SB203580-iodo | - |
Homo sapiens | |
| SB203580-sulfone | - |
Homo sapiens | |
| SB220025 | - |
Homo sapiens | |
| SC68376 | - |
Homo sapiens | |
| SKF86002 | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q16539 | isozyme p38 MAPKalpha | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged p38 MAP kinase | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | nonactivated p38 MAP kinase | Homo sapiens | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| p38 MAP kinase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | cooperative thermal unfolding profile of p38 MAP kinase, overview | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00003 | 0.0006 | pH 7.2, 25°C | Homo sapiens | SB203580 | |
| 0.001 | - |
pH 7.2, 25°C | Homo sapiens | SKF86002 | |
| 0.002 | 0.005 | pH 7.2, 25°C | Homo sapiens | SC68376 | |
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