Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.24 extracted from

  • Spiliotis, M.; Konrad, C.; Gelmedin, V.; Tappe, D.; Brueckner, S.; Moesch, H.U.; Brehm, K.
    Characterisation of EmMPK1, an ERK-like MAP kinase from Echinococcus multilocularis which is activated in response to human epidermal growth factor (2006), Int. J. Parasitol., 36, 1097-1112.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Epidermal growth factor MPK1 is responsive to exogenous host epidermal growth factor, which increases MPK1 phosphorylation Echinococcus multilocularis
hepatocyte host hepatocytes lead to maximal induction of MPK1 phosphorylation Echinococcus multilocularis
serum host serum positively effects MPK1 phosphorylation Echinococcus multilocularis

Application

Application Comment Organism
medicine Echinococcus multilocularis metacestode is able to sense epidermal growth factor from the host which results in an activation of the parasite’s MAP kinase cascade. MPK1 contains all amino acid residues characteristic for MAP kinases, including a conserved TEY motif which identifies the protein as a member of the ERK subfamily of MAP kinases Echinococcus multilocularis

Cloned(Commentary)

Cloned (Comment) Organism
entire reading frame encoding MPK1-A cloned into the pBAD adn pBAD/Thio expression system, heterologous expression in Escherichia coli Echinococcus multilocularis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Echinococcus multilocularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
sequence analysis, Western blot analysis Echinococcus multilocularis

Organism

Organism UniProt Comment Textmining
Echinococcus multilocularis
-
isolate H95
-

Purification (Commentary)

Purification (Comment) Organism
-
Echinococcus multilocularis

Source Tissue

Source Tissue Comment Organism Textmining
larva protein and its phosphorylated form present in the larval stages metacestode and protoscolex during in vitro cultivation and during an infection of the intermediate host Echinococcus multilocularis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myelin basic protein
-
Echinococcus multilocularis ADP + phosphorylated myelin basic protein
-
?

Synonyms

Synonyms Comment Organism
MAP kinase
-
Echinococcus multilocularis
mitogen-activated protein kinase
-
Echinococcus multilocularis
MPK1
-
Echinococcus multilocularis