Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged wild-type and mutant ERK3 in human Hela and T98G cells, and in rat 2 cells, fluorescent immunoaffinity subcellular localization study | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D546V | site-directed mutagenesis, the mutant enzyme is exclusively localized in the nucleus and especially in the Golgi apparatus, not in the cytoplasm, in contrast to the wild-type enzyme | Rattus norvegicus |
K717S | site-directed mutagenesis, the mutant enzyme is exclusively localized in the nucleus and Golgi apparatus, not in the cytoplasm, in contrast to the wild-type enzyme | Rattus norvegicus |
additional information | Construction of several truncation mutants | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cytoplasmic MAPKs, e.g. ERK3, translocate to the nucleus subsequent to their phosphorylation by their activating MAPK kinases, EC 2.7.11.25 | Rattus norvegicus | 5737 | - |
Golgi apparatus | ERK3 is targeted to the Golgi or endoplasmic reticulum as an intermediate compartment during translocation from cytoplasm to nucleus, ERK3 possesses a C-terminally targeting motif for the Golgi apparatus | Rattus norvegicus | 5794 | - |
additional information | subcellular localization of wild-type and mutant enzymes, overview, ERK3 translocates from cytoplasm to the nucleus in a temporally regulated exit from a membraneous organelle, nuclear translocation is cell cycle-dependent, mechanism | Rattus norvegicus | - |
- |
nucleus | cytoplasmic MAPKs, e.g. ERK3, translocate to the nucleus subsequent to their phosphorylation by their activating MAPK kinases, EC 2.7.11.25 | Rattus norvegicus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, about, recombinant ERK3, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P27704 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | MAPKs are phosphorylated by their activating MAPK kinases, EC 2.7.11.25 | Rattus norvegicus |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, about, recombinant ERK3, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ERK3 | - |
Rattus norvegicus |