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Literature summary for 2.7.11.23 extracted from

  • Li, X.; Chatterjee, N.; Spirohn, K.; Boutros, M.; Bohmann, D.
    Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes (2016), Sci. Rep., 6, 21455.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.23

Protein Variants

Protein Variants Comment Organism
additional information knockdown of Cdk12 by expression of hairpin RNA Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Drosophila melanogaster 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [DNA-directed RNA polymerase II] Drosophila melanogaster Cdk12 acts on the Ser2 residue in Pol II CTD heptad repeats ADP + phospho-[DNA-directed RNA polymerase II]
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
S2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [DNA-directed RNA polymerase II] Cdk12 acts on the Ser2 residue in Pol II CTD heptad repeats Drosophila melanogaster ADP + phospho-[DNA-directed RNA polymerase II]
-
 ?

Synonyms

Synonyms Comment Organism
CDK12
-
 Drosophila melanogaster
Pol II kinase
-
 Drosophila melanogaster
RNA polymerase II kinase
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Drosophila melanogaster

General Information

General Information Comment Organism
malfunction cells lacking CDK12 function are viable and able to proliferate. Antioxidant gene expression is compromised in flies with reduced Cdk12 function, which makes them oxidative stress sensitive, Cdk12 knockdown increases sensitivity to oxidative stress Drosophila melanogaster
metabolism the enzyme is involved in the regulation of the Nrf2 signalling pathway Drosophila melanogaster
physiological function Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes. The enzyme engages a global shift in gene expression to switch cells from a metabolically active state to stress-defence mode when challenged by external stress. Cdk12 is not essential for bulk mRNA transcription. Cdk12 may be specifically required for stress activated gene expression. Cdk12 suppresses genes that support metabolic functions in stressed conditions. Cdk12 is a positive regulator of CncC target gene activity. Cdk12 is required for Nrf2 reporter activity in vivo, Cdk12 is critical for the expression of endogenous Nrf2 target genes. Cdk12 promotes stress resistance and overall survival in oxidative stress conditions Drosophila melanogaster