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Literature summary for 2.7.11.23 extracted from

  • Tyagi, S.; Ochem, A.; Tyagi, M.
    DNA-dependent protein kinase interacts functionally with the RNA polymerase II complex recruited at the human immunodeficiency virus (HIV) long terminal repeat and plays an important role in HIV gene expression (2011), J. Gen. Virol., 92, 1710-1720.
    View publication on PubMed
Search for more literature for 2.7.11.23

Activating Compound

Activating Compound Comment Organism Structure
additional information DNA-PK is active only in the presence of DNA, no activity in absence of DNA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + HIV Tat Homo sapiens
-
 ADP + phosphorylated HIV-Tat
-
 ?
ATP + [DNA-directed RNA polymerase] Homo sapiens enzyme DNA-PK phosphorylates all three serine residues, Ser2, Ser5, and Ser7, of C-terminal domain, CTD ADP + phospho-[DNA-directed RNA polymerase]
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HL3T1 cell
-
 Homo sapiens
-
JURKAT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + HIV Tat
-
 Homo sapiens ADP + phosphorylated HIV-Tat
-
 ?
ATP + HIV Tat Tat is an efficient substrate for DNA-PK only in the presence of DNA, three putative target sites of DNA-PK phosphorylation Homo sapiens ADP + phosphorylated HIV-Tat
-
 ?
ATP + [DNA-directed RNA polymerase] enzyme DNA-PK phosphorylates all three serine residues, Ser2, Ser5, and Ser7, of C-terminal domain, CTD Homo sapiens ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
ATP + [DNA-directed RNA polymerase] enzyme DNA-PK phosphorylates all three serine residues, Ser2, Ser5, and Ser7, of C-terminal domain, CTD. Serine residues at positions 2 and 7 are preferred to serine 5 Homo sapiens ADP + phospho-[DNA-directed RNA polymerase]
-
 ?

Synonyms

Synonyms Comment Organism
CTD kinase
-
 Homo sapiens
DNA-dependent protein kinase
-
 Homo sapiens
DNA-PK
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction significant impact of DNA-PK knockdown on HIV transcription Homo sapiens
physiological function DNA-dependent protein kinase DNA-PK is a component of the RNAP II complex involved in HIV transcription, the enzyme interacts functionally with the RNA polymerase II complex recruited at the human immunodeficiency virus (HIV) long terminal repeat and plays an important specific role in HIV gene expression. Phosphorylation of residue Ser5 of the RNAP II CTD is linked with the initiation phase of HIV transcription, while phosphorylation of Ser-2 of the CTD is found to be correlated with the elongation phase of HIV gene expression. The transcription factor (TF) IIH complex does not play any role during the elongation phase of transcription. In the absence of TNF-alpha stimulation, the latent proviruses carry both DNA-PK and RNAP II at extremely low levels. TNF-alpha activation for 3 h results in a sixfold increase in the amount of RNAP II at the promoter, and also an increase in DNA-P level. Higher levels of DNA-PK and RNAP II up to the 5' region of the env gene, but lower levels further into the downstream regions. DNA-PK is involved in both the initiation and elongation phases of transcription, albeit more significantly during elongation. DNA-PK facilitates HIV-1 transcription essentially by phosphorylating the RNAP II CTD, and Tat phosphorylation also contributes significantly to HIV replication Homo sapiens