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Literature summary for 2.7.11.23 extracted from

  • Fulop, K.; Pettko-Szandtner, A.; Magyar, Z.; Miskolczi, P.; Kondorosi, E.; Dudits, D.; Bako, L.
    The Medicago CDKC;1-CYCLINT;1 kinase complex phosphorylates the carboxy-terminal domain of RNA polymerase II and promotes transcription (2005), Plant J., 42, 810-820.
    View publication on PubMed
Search for more literature for 2.7.11.23

Activating Compound

Activating Compound Comment Organism Structure
CYCLINT protein endogenous from Medicago sativa and from Medicago trunculata, the enzyme is organized in the CDKC-1/CYCLINT-1 kinase complex, CYCLINT protein is a specific interactor of the enzyme forming the active kinase complex which localizes to the nucleus Medicago sativa
additional information the level and activity of the enzyme is not cell cycle dependent Medicago sativa

Cloned(Commentary)

Cloned (Comment) Organism
in vitro transcription of CDKC-1, transient co-expression of Myc-tagged CDKC-1 and HA-tagged CYCLINT in Arabidopsis thaliana protoplasts for transcription analysis, expression of CDKC-1 as His-tagged and as GST-tagge protein in Escherichia coli strain BL21(DE3) Medicago sativa

Protein Variants

Protein Variants Comment Organism
additional information the CDKC-1/CYCLINT-1 kinase complex can recombinantly restore the transcription activity of HeLA nuclear cell extracts depleted of endogenous CDK9 kinase complexes Medicago sativa

Inhibitors

Inhibitors Comment Organism Structure
additional information the level and activity of the enzyme is not cell cycle dependent Medicago sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the level and activity of the enzyme is not cell cycle dependent Medicago sativa
-
-
nucleus CDKC-1/CYCLINT-1 kinase complex Medicago sativa 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Medicago sativa the CDKC-1/CYCLINT-1 kinase complex is a positive regulator of transcription in Medicago sativa, the level and activity of the enzyme is not cell cycle dependent ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
 var. genotype A2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged CDKC-1 from Escherichia coli by nickel affinity chromatography, native CDKC-1 partially from cell suspension cultured cells by immunoprecipitation, immobilization of the enzyme on protein A agarose Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Medicago sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [DNA-directed RNA polymerase] CDKC1 phosphorylates the C-terminal YSPTSPS hexapeptide repeat domain CTD of the largest subunit of RNA polymerase II at Ser5, no activity with RNA polymerase II mutant S5A Medicago sativa ADP + phospho-[DNA-directed RNA polymerase]
-
 ?
additional information the CDKC-1/CYCLINT-1 kinase complex is a positive regulator of transcription in Medicago sativa, the level and activity of the enzyme is not cell cycle dependent Medicago sativa ?
-
 ?
additional information the enzyme also performs the reaction of EC 2.7.11.22 Medicago sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
CDKC1
-
 Medicago sativa
More the enzyme is organized in the CDKC-1/CYCLINT-1 kinase complex, see also EC 2.7.11.22 Medicago sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Medicago sativa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Medicago sativa