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Literature summary for 2.7.11.23 extracted from

  • Guilfoyle, T.J.
    A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase II (1989), Plant Cell, 1, 827-836.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.23

Inhibitors

Inhibitors Comment Organism Structure
Bovine serum albumin conjugated to heptapeptide with RNA-polymerase II subunit as substrate Triticum aestivum
heparin
-
 Triticum aestivum
additional information no inhibition by CTP, GTP, UTP, bovine serum albumin alone; no inhibition by EGTA Triticum aestivum
spermidine 3 mM Triticum aestivum
Synthetic peptide multimers of consensus heptapeptide repeat Pro-Thr-Ser-Pro-Ser-Tyr-Ser, with RNA-polymerase II subunit as substrate Triticum aestivum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Triticum aestivum
Mg2+ 2-5 mM Triticum aestivum
Mn2+ activation Triticum aestivum
Mn2+ 2-10 mM, can replace Mg2+ to some degree Triticum aestivum
additional information no activation by Ca2+ Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] Triticum aestivum
-
 ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
 Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein phosphorylated at about 30% the rate of RNA-polymerase II subunit Triticum aestivum ADP + phosphocasein
-
 ?
ATP + synthetic peptides bovine serum albumin conjugated to heptapeptide Triticum aestivum ADP + ?
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
-
 Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates to a lesser extent Thr-residues Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] no substrates are bovine serum albumin and calf thymus histone Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] no substrate is GTP Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates predominantly Ser-residues Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] substrates are RNA-polymerase II subunits of wheat germ, soy bean, pea and human Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] distinct from other protein phosphokinases, transfers about 20 phosphates to the heptapeptide repeats Pro-Thr-Ser-Pro-Ser-Tyr-Ser in C-terminal domain of MW 220000 subunit of RNA-polymerase II Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] no substrates are CTP and UTP Triticum aestivum ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Triticum aestivum

Cofactor

Cofactor Comment Organism Structure
additional information no activation by spermidine and EGTA Triticum aestivum