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Literature summary for 2.7.11.22 extracted from

  • Zheng, F.; Quiocho, F.A.
    New structural insights into phosphorylation-free mechanism for full cyclin-dependent kinase (CDK)-cyclin activity and substrate recognition (2013), J. Biol. Chem., 288, 30682-30692.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.22

Activating Compound

Activating Compound Comment Organism Structure
Pcl10
-
 Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
Pho85-Pcl10 in complex with ATPgammaS, hanging drop vapor diffusion method, using 22-25% (w/v) polyethylene glycol 5000 monomethyl ether, 0.1 M MES, pH 6.4, at 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Gcn4 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Gcn4 protein
-
 ?
ATP + Glc8 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Glc8 protein
-
 ?
ATP + Gsy2 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Gsy2 protein
-
 ?
ATP + Pho4 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Pho4 protein
-
 ?
ATP + Rga2 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Rga2 protein
-
 ?
ATP + Rim 15 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Rim15 protein
-
 ?
ATP + Rvs167 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Rvs167 protein
-
 ?
ATP + Sic1 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Sic1 protein
-
 ?
ATP + Whi5 G1 protein Saccharomyces cerevisiae
-
 ADP + phosphorylated Whi5 G1 protein
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P17157
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon column chromatography and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Gcn4 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Gcn4 protein
-
 ?
ATP + Glc8 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Glc8 protein
-
 ?
ATP + Gsy2 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Gsy2 protein
-
 ?
ATP + Pho4 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Pho4 protein
-
 ?
ATP + Rga2 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Rga2 protein
-
 ?
ATP + Rim 15 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Rim15 protein
-
 ?
ATP + Rvs167 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Rvs167 protein
-
 ?
ATP + Sic1 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Sic1 protein
-
 ?
ATP + Whi5 G1 protein
-
 Saccharomyces cerevisiae ADP + phosphorylated Whi5 G1 protein
-
 ?
additional information the enzyme requires no phosphorylation for kinase activity Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
CDK
-
 Saccharomyces cerevisiae
PHO85
-
 Saccharomyces cerevisiae