Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cks2 | Cks 2 proteins activates Cdk2 | Homo sapiens | |
cyclin E | required for activity of Cdk2, cyclin E is an oncoprotein implicated in breast cancer etiology that triggers the DNA damage response | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
co-expression of His-tagged Cdk2/CycE in insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cks1 | Cks 1 protein inactivates Cdk2, activation of Chk1 leads to inactivation of Cdk2 by promoting phosphorylation-dependent turnover of the Cdk activating phosphatase Cdc25A | Homo sapiens | |
roscovitine | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Cdk2 is inactivated by phosphorylation on Tyr15, Activation of Chk1 leads to inactivation of Cdk2 by promoting phosphorylation-dependent turnover of the Cdkactivating phosphatase Cdc25A. Recombinant His-tagged Cdk2 phosphorylation at Tyr15 by recombinant GST-tagged Wee1 kinase | Homo sapiens |
Purification (Comment) | Organism |
---|---|
co-purification of recombinant His-tagged Cdk2/CycE from insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
epithelial cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
IME cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
MDA-MB-436 cell | - |
Homo sapiens | - |
additional information | breast cancer-derived cell lines expressed Cks2 mRNA at levels two to fourfold higher than HEK-293A and IME cells | Homo sapiens | - |
SUM-149PT cell | - |
Homo sapiens | - |
ZR75-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Rb peptide | from retinoblastoma-associated protein | Homo sapiens | ADP + Rb phosphopeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDK2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | cyclin-dependent kinase subunit 1 and 2 natural overexpression in human mammary epithelial and breast cancer-derived cells, as well as in other cell types, leads to override of the intra-S-phase checkpoint that blocks DNA replication in response to replication stress. Specifically, binding of Cks1 or Cks2 to cyclin-dependent kinase 2 confers partial resistance to the effects of inhibitory tyrosine phosphorylation mediated by the intra-S-phase checkpoint, allowing cells to continue replicating DNA even under conditions of replicative stress, a mechanism contributing to tumor development. Cks protein overexpression does not affect checkpoint signaling through modulation of Cdk2 inhibitory phosphorylation | Homo sapiens |
additional information | Cks1 subunit is involved in SCF-dependent degradation of Cdk inhibitors, while Cks2 subunit is not. Cks1 deficiency is protective against tumor development in genetically engineered mouse models of lymphoma and mammary tumorigenesis | Homo sapiens |