Literature summary for 2.7.11.22 extracted from

Hizli, A.A.; Chi, Y.; Swanger, J.; Carter, J.H.; Liao, Y.; Welcker, M.; Ryazanov, A.G.; Clurman, B.E.
Phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase (2013), Mol. Cell. Biol., 33, 596-604.

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Activating Compound

Activating Compound	Comment	Organism	Structure
cyclin A	activates Cdk2	Homo sapiens
cyclin E	activates Cdk2	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
functional co-expression of CDK2 in 293A, 293T or U2OS cells with myc- or GST-tagged cyclin A or E and with FLAG-tagged human eEF2	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-
additional information	cyclin A-CDK2 shuttles from the cytoplasm to the nucleus and could phosphorylate eEF2 in interphase cells	Homo sapiens	-	-
nucleus	-	Homo sapiens	5634	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + eukaryotic elongation factor 2	Homo sapiens	phosphorylation on Ser595 by Cdk2	ADP + phosphorylated eukaryotic elongation factor 2	-	?
ATP + [elongation factor 2]	Homo sapiens	the enzyme phosphorylates Ser595. Ser595 phosphorylation varies during the cell cycle and is required for efficient Thr56 phosphorylation (by elongation factor 2 kinase) in vivo	ADP + [elongation factor 2]phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + biotin-TVSEESNVLCLSKSPNKHNRLYMKARPFF	-	Homo sapiens	ADP + biotin-TVSEESNVLCLSKpSPNKHNRLYMKARPFF	phosphorylated on Ser595	?
ATP + eukaryotic elongation factor 2	phosphorylation on Ser595 by Cdk2	Homo sapiens	ADP + phosphorylated eukaryotic elongation factor 2	-	?
ATP + eukaryotic elongation factor 2	phosphorylation on Ser595 by Cdk2. Substrate mutant S595A is poorly phosphorylated by cyclin A-CDK2	Homo sapiens	ADP + phosphorylated eukaryotic elongation factor 2	-	?
ATP + [elongation factor 2]	the enzyme phosphorylates Ser595. Ser595 phosphorylation varies during the cell cycle and is required for efficient Thr56 phosphorylation (by elongation factor 2 kinase) in vivo	Homo sapiens	ADP + [elongation factor 2]phosphate	-	?
ATP + [elongation factor 2]	the enzyme phosphorylates Ser595	Homo sapiens	ADP + [elongation factor 2]phosphate	-	?

Synonyms

Synonyms	Comment	Organism
CDK2	-	Homo sapiens
cyclin A-cyclin-dependent kinase 2	-	Homo sapiens
cyclin-dependent kinase 2	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens
cyclin A	cyclin Acyclin-dependent enzyme. Phosphorylation of elongation factor 2 on Ser595 by the cyclin A-dependent enzyme directly stimulates phosphorylation of elongation factor 2 by elongation factor 2 kinase on Thr56	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	S595 phosphorylation may globally couple the cell cycle machinery to regulatory pathways that impact eEF2K activity, eEF2 S595 phosphorylation is increased in mitosis. Cyclin A-CDK2 shuttles from the cytoplasm to the nucleus and could phosphorylate eEF2 in interphase cells	Homo sapiens
physiological function	Ser595 phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase through T56 phosphorylation. S595 phosphorylation varies during the cell cycle and is required for efficient T56 phosphorylation in vivo, T56 phosphorylation inactivates eEF2 and is the only known normal eEF2 functional modification. S595 phosphorylation facilitates T56 phosphorylation by recruiting eEF2K to eEF2.	Homo sapiens

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Release 2023.1 (January 2023)