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Literature summary for 2.7.11.22 extracted from
- Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates (2005), Nature, 434, 104-108.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of mutant Cdk1-as1 with an enlarged ATP binding site | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of Cdk1-As1 mutant with cyclins 5B and 2B in reaction with different substrates, overview | Saccharomyces cerevisiae | |
| 0.003 | - |
Fin1 | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
| 0.046 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 2B | Saccharomyces cerevisiae |  |
| 0.521 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | determination of cyclin specificity of Cdk1 during cell cycle using mutant Cdk1-as1 with an enlarged ATP binding site, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
strains W303 and S288C and derivatives | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| tandem purification of Cdk1 with cyclin B2 or cyclin B5 by affinity chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + ADAQHATPPKKKRKVEDPKDF | a histone peptide substrate | Saccharomyces cerevisiae | ADP + ADAQHAT(P)PPKKKRKVEDPKDF | - |
? | |
| ATP + Fin1 | - |
Saccharomyces cerevisiae | ADP + phosphorylated Fin1 | - |
? | |
| ATP + histone H1 | - |
Saccharomyces cerevisiae | ADP + phosphorylated histone H1 | - |
? | |
| additional information | determination of cyclin specificity of Cdk1 during cell cycle using mutant Cdk1-as1 with an enlarged ATP binding site, overview | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | determination of cyclin specificity of Cdk1 with different enzyme substrates using mutant Cdk1-as1 with an enlarged ATP binding site, overview | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cdk1 | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
Fin1 | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
| 1.9 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 5B | Saccharomyces cerevisiae | |
| 3.15 | - |
ADAQHATPPKKKRKVEDPKDF | pH 7.4, Cdk1-as1/cyclin 2B | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | mutant Cdk1-as1 has an enlarged ATP binding site | Saccharomyces cerevisiae | |
| cyclin B2 | i.e. Clb2 | Saccharomyces cerevisiae | |
| cyclin B5 | i.e. Clb5, specificity mechanism, overview | Saccharomyces cerevisiae | |
| additional information | determination of cyclin specificity of Cdk1 during cell cycle and with different enzyme substrates, overview | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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