General Stability | Organism |
---|---|
the PEST degradation sequence determines the short half-life of cyclin enzyme cofactors | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | translocation to the nucleus | Homo sapiens | 5737 | - |
nucleus | translocation from the cytoplasm | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + retinoblastoma protein | Homo sapiens | i.e. Rb protein | ADP + phosphorylated retinoblastoma protein | - |
? | |
additional information | Homo sapiens | viral K-cyclin has a much longer half-life compared to cellular cyclins because it lacks the PEST degradation sequence, the viral K-cyclin can substitute the cellular cyclins in binding to the cellular CDKs, which is important for the virus development, chimeric K-cyclin-cdks are also translocated to the nucleus, chimeric K-cyclin/cdk6 kinase is constitutively active in BC cells, chimeric K-cyclin-cyclin D2 can act as a CDK | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the PEST degradation sequence determines the short half-life of cyclin enzyme cofactors | Homo sapiens |
Purification (Comment) | Organism |
---|---|
subcellular fractionation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BC-1 cell | primary effusion lymphoma cell line with Kaposi sarcoma-associated herpesvirus-infection, constitutively active K-cyclin/cdk6 kinase | Homo sapiens | - |
BC-2 cell | primary effusion lymphoma cell line with Kaposi sarcoma-associated herpesvirus-infection, constitutively active K-cyclin/cdk6 kinase | Homo sapiens | - |
BC-3 cell | primary effusion lymphoma cell line with Kaposi sarcoma-associated herpesvirus-infection, constitutively active K-cyclin/cdk6 kinase | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone H1 | substrate of e.g. of cheimeric K-cyclin/cdk6 and K-cyclin-cyclin D2 | Homo sapiens | ADP + phosphorylated histone H1 | - |
? | |
ATP + retinoblastoma protein | i.e. Rb protein | Homo sapiens | ADP + phosphorylated retinoblastoma protein | - |
? | |
ATP + retinoblastoma protein | i.e. Rb protein, recombinant GST-tagged substrate, substrate of e.g. of chimeric K-cyclin/cdk6 and K-cyclin-cyclin D2 | Homo sapiens | ADP + phosphorylated retinoblastoma protein | - |
? | |
additional information | viral K-cyclin has a much longer half-life compared to cellular cyclins because it lacks the PEST degradation sequence, the viral K-cyclin can substitute the cellular cyclins in binding to the cellular CDKs, which is important for the virus development, chimeric K-cyclin-cdks are also translocated to the nucleus, chimeric K-cyclin/cdk6 kinase is constitutively active in BC cells, chimeric K-cyclin-cyclin D2 can act as a CDK | Homo sapiens | ? | - |
? | |
additional information | enzyme cdk2, cdk4, and especially cdk6 interact with the KSHV viral K-cyclin, a homologue of cellular cyclin D, in BC3 cells, K-cyclin also interacts with p21Cip1 and p27Kip1 in the cells, chimeric K-cyclin/cdk6 kinase is constitutively active, chimeric K-cyclin-cyclin D2 can act as a CDK | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDK2 | - |
Homo sapiens |
cdk6 | - |
Homo sapiens |
K-cyclin/cdk6 kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
cyclin A | required regulatory subunit of CDKs | Homo sapiens | |
cyclin B1 | required regulatory subunit of CDKs | Homo sapiens | |
cyclin D | required regulatory subunit of CDKs | Homo sapiens | |
cyclin D2 | required regulatory subunit of cdk6 | Homo sapiens | |
cyclin E | required regulatory subunit of CDKs | Homo sapiens | |
additional information | viral K-cyclin has a much longer half-life compared to cellular cyclins because it lacks the PEST degradation sequence, the viral K-cyclin can substitute the cellular cyclins in binding to the cellular CDKs, which is important for the virus development | Homo sapiens |