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Literature summary for 2.7.11.22 extracted from
- Cyclin-dependent kinase 11p110 activity in the absence of CK2 (2003), Biochim. Biophys. Acta, 1624, 98-108.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination of isozyme alpha2-2, expression of FLAG-tagged or HA-tagged CDK11p110 in COS-7 or HEK293 cells, expression of CDK11p110 in Spodoptera frugiperda Sf9 cells via baculovirus infection system, expression of His6-tagged wild-type and mutant CDK11p110 in Escherichia coli strain BL21(DE3), optimization of an expression system producing enzyme free of CK2, overview | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S227A | site-directed mutagenesis, phosphorylation site mutant | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, recombinant CDK11p110, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | CDK11p110 is involved in transcription and RNA processing | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P21127 | alpha2-2 isozyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | CDK11p110 is phosphorylated at Ser227 by the serine/threonine kinase CK2 | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged wild-type and mutant CDK11p110 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, co-purification of CK2, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + histone H1 | substrate of CDK11p110 | Homo sapiens | ADP + phosphorylated histone H1 | - |
? | |
| additional information | CDK11p110 is involved in transcription and RNA processing | Homo sapiens | ? | - |
? | |
| additional information | CDK11p110 interacts with Hsp90, and the serine/threonine kinase CK2, the C-terminal domain of the largest subunit of RNA polymerase II is no substrate of CDK11p110, but of CK2 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, recombinant CDK11p110, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDK11p110 | - |
Homo sapiens |
| cyclin-dependent kinase 11p110 | - |
Homo sapiens |
| PITSLRE | formerly | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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